复配菌对含蜡原油含蜡量及其黏度作用分析

Effect of Compound Bacteria on Wax Content and Viscosity of Waxy Crude Oil

微生物能改善含蜡原油的流动性,提高运输效率。将已培养的枯草芽孢杆菌6#和铜绿假单胞菌3#按2∶3的体积比复配培养,并优化其生长条件。采用DSC、偏光显微镜、HAKEE流变仪和界面张力仪对复配菌与含蜡原油的作用效果进行分析。经复配菌处理后,含蜡原油的析蜡点降低了2.23℃,析蜡高峰点降低了2.71℃,原油含蜡量(原油中蜡质量分数)降低51.64%;蜡晶结构发生了明显变化;37℃下测得原油表观黏度降低了64.72%;复配菌对液体石蜡的乳化指数为55.39%,液体石蜡培养基的表面张力从79.89 mN/m降低至38.83 mN/m。与单菌相比,复配菌在除蜡降黏方面效果更为优异,应用前景更为广阔。

Microbes can improve the fluidity of waxy crude oil and increase the efficiency of transportation.The cultured Bacillus subtilis 6#and Pseudomonas aeruginosa 3#were mixed and cultured in a ratio of 2∶3,the growth conditions were optimized.DSC,polarizing microscope,HAKEE rheometer and interfacial tensiometer were used to analyze the effect of compound bacteria and waxy crude oil.The results showed that:after treatment with compound bacteria,the wax precipitation point of waxy crude oil decreased by 2.23℃,the peak point of wax precipitation decreased by 2.71℃,and the wax content of crude oil decreased by 51.64%;the wax crystal structure changed significantly;the apparent viscosity of crude oil decreased by 64.72% at 37℃.In addition,the emulsification index of the compound bacteria on liquid paraffin was 55.39%,and the surface tension of the liquid paraffin culture medium was reduced from 79.89 mN/m to 38.83 mN/m.Compared with single bacteria,compound bacteria are more effective in removing wax and reducing viscosity,and have wider application prospects.