百香果茎段愈伤组织诱导分化研究

Study on Callus Induction and Differentiation of Passiflora edulis Stems

以百香果茎段为试验材料,采用不同的消毒方法,研究不同处理对百香果消毒效果的影响,并筛选出愈伤组织诱导分化的最佳培养基配方。结果表明:百香果茎段使用洗衣粉溶液浸泡5 min,结合75%乙醇30 s+HgCl212 min的消毒效果最好,污染率最低,仅20%;愈伤组织诱导与分化的最适培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,诱导率为70%,分化率为40%。

Taking stems of Passiflora edulis as explants,the disinfection effects of different disinfection methods on stems were studied,and the optimum medium for callus induction and differentiation was selected.The results showed that the most suitable disinfection scheme was to soak the stems in solution of laundry powder for 5 min and disinfect the stems with 75%alcohol for 30 s+2%HgCl2 for 12 min.The optimum medium for callus induction and differentiation was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,the induction rate was 70%,the differentiation rate was 40%.