生存素调控季节性变应性鼻炎患者鼻黏膜CD4+T细胞凋亡的机制

Mechanism of survivin regulating apoptosis of nasal mucosal CD4+T cells in patients with seasonal allergic rhinitis

目的探讨生存素(Survivin)调控季节性变应性鼻炎(seasonal allergic rhinitis,SAR)患者鼻黏膜CD4+T细胞凋亡的机制。方法选择2018年5月~2019年5月西安市中医院收治的50例SAR患者为研究组,同期收治的50例单纯进行鼻中隔检查者为对照组,比较两组鼻黏膜组织中Survivin浓度。分离50例SAR患者鼻黏膜CD4+T细胞,将CD4+T细胞分为空白对照组、阴性对照组、Survivin过表达组,空白对照组不作任何处理,阴性对照组转染无关干扰序列,Survivin过表达组转染Survivin过表达质粒,采用流式细胞术检测各组CD4+T细胞凋亡率,采用Western blot检测各组CD4+T细胞中Survivin、磷脂酰肌醇-3-激酶(phosphoinositide3-kinase,PI-3K)、磷酸化丝氨酸苏氨酸激酶(phosphorylated ser ine threonine kinase,p-AKT)蛋白表达量。结果研究组鼻黏膜组织中Survivin浓度较对照组明显升高(P<0.05);Survivin过表达组CD4+T细胞中Survivin蛋白表达量明显高于空白对照组、阴性对照组(P<0.05);阴性对照组与空白对照组CD4+T细胞中Survivin蛋白表达量比较差异无统计学意义(P>0.05);Survivin过表达组CD4+T细胞凋亡率明显低于空白对照组、阴性对照组(P<0.05);阴性对照组与空白对照组CD4+T细胞凋亡率比较差异无统计学意义(P>0.05);Survivin过表达组CD4+T细胞中PI3K、p-AKT蛋白表达量明显高于空白对照组、阴性对照组(P<0.05);阴性对照组与空白对照组CD4+T细胞中PI3K、p-AKT蛋白表达量比较差异无统计学意义(P>0.05)。结论Survivin可能通过激活PI3K/AKT信号通路抑制SAR患者鼻黏膜CD4+T细胞凋亡。

Objective To investigate the mechanism of Survivin regulating the apoptosis of CD4 T cells in nasal mucosa of patients with seasonal allergic rhinitis(SAR).Methods 30 SAR patients(study group)and 50 patients with nasal septum examination(control group)in Xi'an Hospital of Traditional Chinese Medicine from May 2018 to May 2019 were selected;The concentration of Survivin in nasal mucosa was compared between groups.CD4 T cells in nasal mucosa in study group were isolated,and divided into three groups for different treatments,blank control group,negative control group and Survivin over expression group.Control group received no treatment,negative control group was transfected with non-interference sequences,and the Survivin over expressed group was transfected with Survivin over expressed plasmid.The apoptosis rate of CD4 T cells in each group was detected by flow cytometry,and the protein expression of survivin,phosphatidylinositol-3-kinase(PI3K),phosphorylated serine threonine kinase(p-Akt)in CD4 T cells in each group was detected by Western blot.Results Survivin concentration in nasal mucosa of study group was significantly higher than that of control group(P<0.05).The expression of Survivin protein was significantly higher in Survivin over expression group than that in the blank control group and the negative control group(P<0.05),while no statistic difference was found between blank control group and the negative control group(P>0.05).The apoptosis rate of CD4 T cells was significantly lower in Survivin over expression group than that in the blank control group and the negative control group(P<0.05),while no statistic difference was found between blank control group and the negative control group(P>0.05).The expression of PI3K and p-AKT protein was significantly higher in Survivin over expression group than that in blank control group and negative control group(P<0.05),while no statistic difYerence was found between blank control group and the negative control group(P>0.05).Conclusion Survivin may inhibit the apoptosis of nasal mucosa CD4 T cells in patients with seasonal allergic rhinitis by activating PI3K/AKT signaling pathway.