扎里奴思方水提液在大鼠血浆、胆汁、尿液和粪便中代谢产物鉴定

Identification of metabolites of Zhali Nusi Prescription in rat plasma,bile,urine and feces after oral administration

研究扎里奴思方在大鼠体内的代谢产物。采用超高效液相色谱-线性离子肼-静电轨道肼串联质谱联用方法(UHPLC-LTQ-Orbitrap-MS)对扎里奴思方在大鼠体内的代谢产物进行分析鉴定。采用ACQUITY UPLC BEH T3色谱柱(2.1 mm×100 mm,1.7μm),以0.1%甲酸水(A)-乙腈(B)为流动相进行梯度洗脱,在电喷雾质谱负离子模式下对生物样品进行分析。建立了大鼠给药后生物样品的分析方法,共鉴定了8个原型成分和36个代谢产物。结果表明,扎里奴思方主要成分在大鼠体内的代谢途径主要包括甲基化、葡萄糖醛酸化、硫酸化等。为进一步探讨扎里奴思方体内过程,阐明其功效物质基础提供依据。

This study was designed to determine the metabolites of Zhali Nusi Prescription(ZLNSP)in rats.The ultra-high performance liquid chromatography-LTQ Orbitrap mass spectrometric(UHPLC-LTQ-Orbitrap-MS)and mass defect filter techniques were applied to analyze the metabolites of ZLNSP in rat plasma,bile,urine and feces.The biological samples were analyzed by ACQUITY UPLC BEH T3 column(2.1 mm×100 mm,1.7μm),with 0.1%formic acid water(A)-acetonitrile(B)as mobile phase,and the biological samples were analyzed in negative ion mode by electrospray ionization mass spectrometry(ESI-MS).An analytical method for biological samples of rats was established,and 8 prototype components and 36 metabolites were identified.The results showed that the metabolic pathways of the main components of ZLNSP in rats included methylation,glucuronidation,sulfation and so on.It provi-ded information for the therapeutic effect of ZLNSP in vivo.