基于枳实薤白桂枝汤阐释“胃络通心”理论及其潜在分子作用机制

Interpretation of Theory of Weiluo Tongxin and Its Potential Molecular Mechanism Based on Zhishi Xiebai Guizhitang

中医理论内涵的科学阐释是中医学自身发展的重要内容,将中医经典理论与现代科学技术有机结合是有效途径之一。在系统梳理中医"胃络通心"理论的基础上,笔者选择经典名方枳实薤白桂枝汤为例,借助中医药整合药理学研究平台V2.0(TCMIP V2.0)的分子挖掘技术,分析枳实薤白桂枝汤防治胃心痛的作用机制,得到了枳实薤白桂枝汤的220种化学成分,涉及674个靶标,其中直接作用于心绞痛和胃食管反流病的核心靶标有12个,包括胰岛素(INS),甘油醛-3-磷酸脱氢酶(GAPDH),丝氨酸/苏氨酸蛋白激酶1(AKT1),肿瘤蛋白p53(TP53),白蛋白(Alb),丝裂原活化蛋白激酶3(MAPK3),白细胞介素-6(IL-6),90 kDa热休克蛋白αA1(HSP90AA1),cAMP反应元件结合蛋白1(CREB1),雌激素受体1(ESR1),肿瘤坏死因子(TNF),半胱氨酸蛋白酶-3(Caspase-3),涉及相关疾病的TNF信号通路,磷脂酰肌醇-3激酶/蛋白激酶B(PI3K/Akt)信号通路,IL-17信号通路,低氧诱导因子-1(HIF-1)信号通路等125条通路,这些均是"胃络通心"理论的生物学基础。中医经典理论的梳理结合代表方药的分子机制挖掘,可为中医理论的科学内涵研究提供示范和借鉴。

The scientific interpretation of the connotation of traditional Chinese medicine(TCM) theory is an important part of the development of TCM. Combined ancient classic theories with modern science and technology is a new path for the innovative development of TCM theory. Based on this,taking Zhishi Xiebai Guizhitang as an example, the molecular mining technology of integrative pharmacology-based research platform of TCM V2.0(TCMIP V2.0)was used to analyze the mechanism of Zhishi Xiebai Guizhitang in preventing and treating gastropathic stomachache. A total of 220 chemical components in Zhishi Xiebai Guizhitang were obtained,and 674 targets were involved,of which 12 core targets directly affected angina pectoris and gastroesophageal reflux disease, including insulin(INS), glyceraldehyde-3-phosphate dehydrogenase(GAPDH),RAC-α serine/threonine-protein kinase 1(AKT1),tumour protein p53(TP53),albumin(Alb),mitogen-activated protein kinase 3(MAPK3),interleukin-6(IL-6),etc. And tumor necrosis factor(TNF)signaling pathway,phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)signaling pathway,IL-17 signaling pathway, hypoxia-inducible factor-1(HIF-1)signaling pathway and other 121 pathways involved in these related diseases. All of these are the biological basis of Weiluo Tongxin theory. Combing the classical theories of TCM combined with the exploring the molecular mechanism of representative prescriptions can provide a demonstration and reference for the scientific connotation research of TCM theory.