转录组测序揭示不同生长速度番鸭胸肌组织的基因表达差异

Transcriptome Sequencing Reveals Gene Expression Differences in Breast Muscle Tissue of Muscovy Ducks with Different Growth Rates

【目的】利用转录组测序技术分析不同生长速度番鸭的基因表达差异,挖掘影响番鸭生长性能的基因与信号通路,为阐明生长性能差异的遗传机制提供理论基础。【方法】选取不同生长速度两尾样番鸭的胸肌组织进行转录组测序,采用无参考基因组的分析手段对测序结果进行分析,获得差异表达基因(Differentially expressed genes,DEGs)。利用Blast2 GO软件对DEGs进行功能富集分析,利用KAAS网站进行DEGs的通路富集分析。【结果】转录组测序分析结果显示,快速生长型番鸭与缓慢生长型番鸭相比共有186个显著DEGs,其中49个表达量上调,137个表达量下调。这些DEGs主要富集在磷脂酰肌醇三激酶-丝氨酸/苏氨酸激酶(PI3K-AKT)信号通路、丝裂原活化蛋白激酶(MAPK)信号通路和腺苷酸激活蛋白激酶(AMPK)信号通路。【结论】PI3K-AKT、MAPK、AMPK这3条信号通路是控制番鸭机体生长发育的重要调节信号通路。

【Objective】Transcriptome sequencing technology was used to analyze the gene expression differences in Muscovy ducks with different growth rates,and to explore the genes and signaling pathways that affect the growth performance of Muscovy ducks,providing a theoretical basis for elucidating the genetic mechanism of the differences in growth performance.【Method】The breast muscle tissues of two-tailed Muscovy ducks with different growth rates were selected for transcriptome sequencing,and the sequencing results were analyzed by using analysis methods without reference genomes to obtain differentially expressed genes(DEGs).Blast2 GO software was used to analyze the functional enrichment of DEGs,and the KEGG pathways enrichment of DEGs was analyzed with the KAAS website.【Result】Transcriptome sequencing analysis shows that there are 186 significant DEGs in the fast-growing Muscovy ducks and the slow-growing Muscovy ducks,of which 49 up-regulated and 137 down-regulated.These DEGs are mainly enriched in phosphatidylinositol 3-kinase-serine/threonine kinase(PI3K-AKT)signaling pathway,mitogen-activated protein kinase(MAPK)signaling pathway and adenylate-activated protein kinase(AMPK)signaling pathway.【Conclusion】The PI3KAKT,MAPK and AMPK are important regulatory signaling pathways that control the growth and development of Muscovy ducks.