毒理学数据可靠性评价工具在谷氨酸及其盐神经毒性危害评估中应用

Application of toxicology data reliability assessment method,a toxicological data reliability evaluation tool,in the neurotoxic hazard assessment of glutamate acid and its salts

目的评价谷氨酸及其盐神经毒性数据的可靠性并给出使用建议,为谷氨酸及其盐类危害评估提供科学保证;在使用过程中检验毒理学数据可靠性评价工具(toxicology data reliability assessment method,TRAM),为进一步完善工具提供参考。方法使用TRAM对通过系统综述中检索文献的方法筛选出的60篇谷氨酸及其盐神经毒性相关文献进行可靠性评价。根据文献所涉及的数据类型(动物试验数据、人类数据)进入相应的评价流程,按照评分要素及建议分值对各个评价指标逐条打分。计算每条得分与对应权重的乘积之和,除以各类数据总分得出质量分数,最后根据质量分数判定可靠性等级并给出使用建议。其中,每篇文献的评价工作均由相关领域的两名人员独立完成。结果最终12篇文献可靠性等级评价为"高",建议优先使用;43篇文献可靠性等级评价为"中",可以使用;5篇文献可靠性等级评价为"低",不建议使用。结论TRAM的评价指标兼顾了报告质量和方法学质量,且填补了其他工具涉及人类毒理学资料可靠性评价方法的空白。TRAM对食品安全风险评估工作具有更好的适用性,为更加客观、科学地开展危害识别以及风险评估工作提供了方法和手段。

Objective This paper aims to evaluate data reliability of the neurotoxic hazard assessment of glutamate and its salts and provide recommendations,as well as to improve toxicology data reliability assessment method(TRAM)via trial application.Methods TRAM was used to evaluate the reliability of 60 articles which were selected by the method of systematic review documentation retrieval.The evaluation was based on types of toxicological data involved in each paper(laboratory animal data or human data)and they were scored by reliability criteria.The quality percentage was obtained via calculations to judge reliability categories and give recommendations.It’s necessary to note that the evaluation of each paper was independently completed by two persons in related fields.Results After three rounds of evaluation,the reliability of 12 articles were evaluated as"high"and recommended for priority use.The reliability of 43 articles was rated as"moderate"and can be used.The reliability of 5 articles was evaluated as"low"and not recommended to use.Conclusion TRAM takes both reporting quality and methodological quality into consideration,especially including human data reliability evaluation method which is absent in the other toxicology data reliability assessment tools.TRAM is more suitable for food safety risk assessment.It provides a better objective and scientific guarantee for hazard identification and risk assessment.