高压氧通过调节HIF-1α对卵巢癌SKOV-3细胞恶性生物学行为的影响

Effects of hyperbaric oxygen on the malignant biological behaviors of SKOV-3 ovarian cancer cells by regulating HIF-1α

目的探究高压氧(HBO)通过调节缺氧诱导因子-1α(HIF-1α)对卵巢癌SKOV-3细胞恶性生物学行为的影响及其作用机制。方法细胞培养完成后,首先将卵巢癌SKOV-3细胞分为对照组和HBO组,初步分析HBO对卵巢癌SKOV-3细胞生物学行为的影响以及对HIF-1α表达的影响。然后将余下的SKOV-3细胞按照随机数字表法分为3组:NC组、HBO组和HBO+HIF-1α组。HBO组在HBO条件下培养,HBO+HIF-1α组在HBO处理前通过转染过表达HIF-1α。采用qPCR、Western blotting、CCK-8、克隆形成、细胞划痕、Transwell实验检测HBO处理后HIF-1α对卵巢癌SKOV-3细胞恶性生物学行为的影响。结果在HBO环境中培养24 h后,卵巢癌SKOV-3细胞的增殖、克隆形成、迁移和侵袭能力均受到明显抑制(P<0.05),并且HIF-1αmRNA和蛋白水平也显著降低(P<0.05)。HBO组SKOV-3细胞的增殖、克隆形成、迁移、侵袭和上皮-间充质转化(EMT)能力均显著低于NC组(P<0.05);而HBO+HIF-1α组的上述指标均显著高于HBO组(P<0.05)。过表达HIF-1α部分逆转HBO对SKOV-3细胞增殖、迁移、侵袭和EMT(P<0.05)。结论HBO可以在体外通过抑制HIF-1α的表达发挥对卵巢癌SKOV-3细胞的抑制作用。

Objective To explore the effects and mechanism of hyperbaric oxygen(HBO)on the malignant biological behaviors of ovarian cancer cells by regulating hypoxia inducible factor-1α(HIF-1α).Methods After cell culture,the SKOV-3 ovarian cancer cells were divided into control group and HBO group.The effects of HBO on the biological behaviors of SKOV-3 cells and the expression of HIF-1αwere preliminarily analyzed.Then the rest of the SKOV-3 cells were divided into three groups:NC group,HBO group,and HBO+HIF-1αgroup,according to random number table.The HBO group was cultured by using HBO;the cells in the HBO+HIF-1αgroup overexpressed their HIF-1αby transfection before HBO treatment.The effects of HIF-1αon malignant biological behaviors of SKOV-3 cells after HBO treatment were detected by quantitative polymerase chain reaction(qPCR),Western blotting,cell counting kit-8(CCK-8),clone formation,cell scratch assay,and Transwell migration assay.Results After 24 h of culturing with HBO,the proliferation,cloning,migration,and invasion abilities of SKOV-3 cells were significantly inhibited(P<0.05),and the levels of HIF-1αmRNA and protein also decreased significantly(P<0.05).The cell proliferation,cloning,migration,invasion,and epithelial-mesenchymal transition(EMT)abilities of the HBO group were significantly lower than those of NC group(P<0.05),while the above indicators of the HBO+HIF-1αgroup were significantly higher than those of the HBO group(P<0.05).The overexpression of HIF-1αwould partially reverse the effects of HBO on the proliferation,migration,invasion,and EMT of SKOV-3 cells.Conclusion HBO can exert an inhibitory effect on SKOV-3 ovarian cancer cells by inhibiting the expression of HIF-1αin vitro.