高压氧处理对脑动脉粥样硬化大鼠炎性因子及氧化应激水平的抑制作用

Inhibitory effects of hyperbaric oxygen on inflammation and oxidative stress level in rats with cerebral atherosclerosis

目的探究高压氧(HBO)对脑动脉粥样硬化大鼠炎性因子和氧化应激水平的影响。方法6周龄雄性SD大鼠30只,饲养1周后,按照数字表法随机分为对照组、模型组和HBO组,每组10只。模型组和HBO组大鼠均采取高脂饮食联合N-硝基-L-精氨酸甲酯(L-NAME)喂养,构建大鼠脑动脉粥样硬化模型;HBO组进一步采取HBO处理。治疗结束后取各组大鼠血清,检测血清中血脂、炎性因子及氧化应激水平。结果处理30 d后,HBO组脑组织中炎性细胞浸润程度明显降低。与对照组比较,模型组大鼠血清中三酰甘油(TG)、总胆固醇(TC)水平及动脉硬化指数(AI)明显升高,而高密度脂蛋白胆固醇(HDL-C)明显降低(P<0.01)。与模型组比较,HBO组大鼠TG、TC及AI明显降低,而HDL-C明显升高(P<0.05)。与对照组比较,模型组大鼠血清中促炎因子TNF-α、IL-1β及IL-6水平明显升高,而抗炎因子IL-10水平明显降低(P<0.01);与模型组比较,HBO组TNF-α、IL-1β及IL-6水平明显降低,而IL-10水平明显升高(P<0.05)。与对照组相比,模型组大鼠血清中SOD及GSH-Px明显降低,MDA明显升高(P<0.01)。经HBO处理后,SOD和GSH-Px明显升高,MDA明显降低(P<0.05)。结论HBO处理可改善脑动脉粥样硬化大鼠的血脂水平,并抑制其氧化应激和炎性反应。

Objective To explore the effects of hyperbaric oxygen(HBO)on inflammation and oxidative stress level in rats with cerebral atherosclerosis.Methods A total of 30 six-week-old male Sprague Dawley(SD)rats,after being fed for one week,were divided into control group,model group,and HBO group with 10 rats in each group,according to random number table method.The rats in the model group and the HBO group were fed with a high-fat diet combined with L-nitro-arginine methyl ester(L-NAME).Those in the HBO group received HBO treatment.After treatment,all the rats’serum was collected to assess the levels of blood lipid,inflammation factors,and oxidative stress.Results After 30 days of treatment,the infiltration degree of inflammatory cells in brain tissues of the HBO group significantly decreased.Compared with the control group,the serum levels of triglyceride(TG),total cholesterol(TC),and atherosclerosis index(AI)in the model group significantly increased,while high-density lipoprotein-cholesterol(HDL-C)significantly decreased(P<0.01).Compared with the model group,the serum levels of TG,TC,and AI in the HBO group significantly decreased,while HDL-C significantly increased(P<0.05).Compared with the control group,the serum levels of pro-inflammatory factors including TNF-α,IL-1β,and IL-6 in the model group significantly increased,while anti-inflammatory factor IL-10 significantly decreased(P<0.01).Compared with the model group,the levels of TNF-α,IL-1β,and IL-6 in HBO group significantly decreased,while IL-10 significantly increased(P<0.05).Compared with the control group,the serum levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the model group significantly decreased,while malondialdehyde(MDA)significantly increased(P<0.01).After HBO treatment,SOD and GSH-Px levels significantly increased,while MDA significantly decreased(P<0.05).Conclusion HBO treatment can improve the level of blood lipid and inhibit oxidative stress and inflammatory response in rats with cerebral atherosclerosis.