布鲁杆菌S2株通过JNK-p53信号通路诱导小鼠BV-2小胶质细胞凋亡

Brucella suis vaccine strain S2 induces apoptosis of murine BV-2 microglia cells via JNK-p53 signaling pathway

目的研究布鲁杆菌S2株诱导BV-2小胶质细胞凋亡的机制,以发现神经型布鲁杆菌病药物治疗的新靶点。方法用布鲁杆菌S2株分别侵染BV-2小胶质细胞0、3、6、12、24 h,Western blot和RT-qPCR技术检测p-JNK、p53蛋白和mRNA表达水平,流式细胞术检测细胞凋亡,细胞免疫荧光技术检测p-JNK蛋白核转运。结果布鲁杆菌S2株能促进BV-2小胶质细胞p-JNK、p53蛋白及mRNA表达,并增加p-JNK蛋白核转运。布鲁杆菌S2株能诱导BV-2小胶质细胞凋亡。结论布鲁杆菌S2株通过激活BV-2小胶质细胞JNK,促进p53表达,诱导细胞凋亡。

Objective To investigate the potential mechanism by which Brucella suis vaccine strain S2 induces the apoptosis of BV-2 microglia cells,and to discover new protein targets for neurobrucellosis treatment.Methods BV-2 microglia cells were treated with Brucella suis vaccine strain S2 for 0,3,6,12 and 24 h.Western blot assay and RT-qPCR were performed to detect the expression of p-JNK and p53 at protein and mRNA levels in BV-2 microglia cells.Cell apoptosis was measured by flow cytometry.Immunofluorescence was used to analyze nuclear p-JNK.Results Brucella suis vaccine strain S2 could promote the expression of p-JNK and p53 at both protein and mRNA levels and increase nuclear p-JNK in BV-2 microglia cells.Moreover,it could also induce the apoptosis of BV-2 microglia cells.Conclusions Brucella suis vaccine strain S2 could promote the apoptosis of BV-2 microglia cells through activating JNK and promoting p53 expression.