超高效液相色谱法测定保健食品中脱氢表雄甾酮的不确定度评定

Uncertainty evaluation on determination of dehydroepiandrosterone in health food by ultra high performance liquid chromatography

目的评定超高效液相色谱法测定保健食品中脱氢表雄甾酮(dehydroepiandrosterone,DHEA)含量的不确定度。方法依据JJF1059.1–012《测量不确定度评定与表示》,分析实验过程DHEA含量测定的不确定度来源。通过建立数学模型以量化不确定度分量,计算合成不确定度及扩展不确定度。结果本研究测得保健食品中DHEA的含量为(85.6±3.70) g/kg,扩展不确定度为3.70 g/kg(k=2)。结论 UPLC法测定DHEA的不确定度主要来源于标准溶液的制备和拟合,其次为样品的重复测定和前处理过程引入的不确定度,其他因素的影响相对较小。

Objective To evaluate the uncertainty of determination of DHEA in health food by ultra high performance liquid chromatography(UPLC). Methods According to the JJF1059.12-012 Chinese regulation on the valuation and expression of uncertainty in measurement, the sources of uncertainty in the determination of DHEA content in the experimental process were analyzed. Through the establisof hment mathematical models to quantify the component of uncertainty, the combined uncertainties and expanded uncertainties were calculated. Results The content of DHEA in health foods in this study was(85.6±3.70) g/kg, and the accompanying expanded uncertainty was 3.70 g/kg(k=2). Conclusions The main sources of uncertainty in the determination by UPLC are preparation of standard solution and the standard curve fitting, followed by measurement repeatability and the sample pretreatment, and the influence of other factors are relatively small.