梁汾醋对小鼠免疫增强作用及体外抗氧化活性

Immunoenhancement and in vitro antioxidant activity of Liangfen vinegar in mice

目的测定梁汾醋中的主要成分及其含量,并探究其对小鼠的免疫增强作用和体外抗氧化活性。方法通过比较低剂量与高剂量梁汾醋对小鼠免疫器官的脏器指数、NK细胞活性、淋巴细胞转化功能、迟发型变态反应和细胞因子变化等的影响,评价梁汾醋对小鼠免疫功能的作用。通过1,1-二苯基-2-苦肼基(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、 2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS]自由基和羟基自由基清除实验,观察梁汾醋的体外抗氧化活性。结果 2个剂量的梁汾醋对小鼠的体重、脾脏指数和胸腺指数均无显著影响,但高剂量组能够显著提高小鼠的NK细胞活性、脾淋巴细胞的转化能力,有效缓解迟发型变态反应,促进细胞因子IL-10、IL-12的释放,增加免疫球蛋白的水平;梁汾醋表现出显著的清除DPPH自由基以及羟基自由基的能力,在一定浓度下清除率可达到100%,对于ABTS自由基也有较好的清除率,在浓度为120μg/mL时,清除率达到76.73%±3.28%,并与浓度呈现量效关系。结论摄入一定量的梁汾醋具有增强小鼠免疫功能的作用,且具有良好的抗氧化效果。

Objective To determine the main components of Liangfen vinegar and its content, and to explore its immune-enhancing effect on mice and antioxidant activity in vitro. Methods By comparing the effects of low dose and high dose Liangfen vinegar on the organ index, NK cell activity, lymphocyte transformation function, delayed allergic reaction and cytokine changes in mouse immune organs, the effects of Liangfen vinegar on mouse immune function were evaluated. The antioxidant activity of Liangfen vinegar was observed in vitro by scavenging1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical, 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),(ABTS) free radical and hydroxyl free radical and hydroxyl radical scavenging experiments. Results Two doses of Liangfen vinegar had no significant effect on the weight, spleen index, and thymus index of mice. The high-dose group could significantly improve the NK cell activity and the transformation ability of splenic lymphocytes in mice, and effectively alleviate the late abnormal response, promote the release of cytokines IL-10 and IL-12 and increase the level of immunoglobulin. Liang fen vinegar showed significant scavenging ability of DPPH free radical and hydroxyl radical, and the scavenging rate could reach 100% under certain concentration. ABTS free radical scavenging rate was also better, the clearance rate was 76.73%±3.28% at the concentration of 120 μg/mL, and showed a dose-effect relationship with the concentration. Conclusion Intake of a certain amount of Liangfen vinegar can enhance the immune function of mice, and has a good antioxidant effect.