睾丸酮丛毛单胞菌QYY降解喹啉的转录组分析

Study of transcriptome analysis of quinoline degradation by Comamonas tesotseroni QYY

以分离出的一株喹啉降解菌睾丸酮丛毛单胞菌QYY为实验对象,利用转录组测序技术,对喹啉质量浓度为0(C组)、100 mg/L降解6h(Q-100组)及400 mg/L降解7h(Q-400组)条件下的菌株进行了测序分析,研究了睾丸酮丛毛单胞菌对喹啉的降解途径和相关基因.结果表明:与对照C组相比,Q-100和Q-400组中分别有上调基因606和915个、下调基因510和685个.其中,参与喹啉降解的bphB基因在两组实验组中均显著上调,ligABC基因在Q-100组中显著上调.此外差异基因主要富集在KEGG数据库中ABC转运系统与能量代谢中,这与底物的识别转运、信号转导、基因表达调控及能量提供有关.

Quinoline is a typical of N-heteroaromatic compound widely used,which is toxic and difficult to degrade.There are few reports on study quinoline degradation pathway and related genes by Comamonas testosteroni.A strain of quinoline-degrading bacteria Comamonas testosterone QYY isolated from a wastewater treatment plant was used as the experimental object in this study.Transcriptome sequencing was performed on the strains degraded by quinoline concentration of 0(C),100 mg/L for 6 h(Q-100)and 400 mg/L for 7 h(Q-400).The results showed that,compared with C,there were 606 and 915 up-regulated genes and 510 and 685 down-regulated genes in the Q-100 and Q-400 groups,respectively.Among them,the bphB gene involved in quinoline degradation was significantly up-regulated in both experimental groups,and the ligABCgene was significantly up-regulated in the Q-100 group.In addition,the differential genes were mainly concentrated in the ABC transport system and energy metabolism in KEGG,which were related to the recognition and transport of substrates,signal transduction,gene expression regulation and energy supply.