甜瓜细菌性果斑病带菌种子的处理方法研究

Study on the treatment methods of melon seed with bacterial fruit blotch(BFB)

以携带细菌性果斑病菌(Acidovoras avenae subsp.citrulli)的甜瓜种子为材料,分别进行70℃、75℃、80℃干热灭菌、包衣剂(卫福Vitavax)、70℃干热灭菌+包衣剂5种方式的处理,并利用特异引物(BX-L1∕BX-S-R2)对细菌性果斑病菌进行PCR检测,以期探索一种既不影响种子发芽率,又能有效杀灭种带细菌性果斑病菌的最佳处理方式。结果表明:除70℃干热灭菌+包衣剂处理外,其他4种处理的种子均能检测到279 bp的目的条带;实时定量PCR结果显示,5种处理的种子细菌性果斑病菌带菌量均有不同程度的降低,其中以70℃干热灭菌+包衣剂处理效果最佳。活菌分离结果表明,只能从70℃干热灭菌的种子上分离到细菌性果斑病菌。种子发芽试验结果显示,75℃和80℃干热处理对种子发芽势和发芽率有一定影响,其他处理均无影响。

In order to explore a kind of best treatment method which does not affect the seed germination rate,but also can effectively kill the BFB pathogen(Acidovoras avenae subsp.citrulli),muskmelon seeds carrying BFB pathogen were treated with 5 methods,namely,70℃,75℃,80℃dry heat sterilization,coating agent(Vitavax),70℃dry heat sterilization+coating agent,and the specific primers(BX-L1∕BX-S-R2)were used to detect BFB pathogen.The results showed that the 279 bp target band could be detected in all the treatments except 70℃dry heat sterilization+coating agent treatment.Quantitative real-time PCR results showed that the amount of BFB pathogen in the 5 treatments decreased in different degrees,and the best treatment was 70℃dry heat sterilization+coating agent.The living pathogen isolation results showed that BFB pathogen could only be isolated from seeds sterilized by dry heat at 70℃.The seed germination results showed that 75℃and 80℃dry heat treatment had effects on seed germination potential and germination rate,while other treatments had no effects.