SERPINE1在结直肠癌中的表达及其预后意义

Expression of SERPINE1 and its prognostic significance in colorectal cancer

目的通过生物信息学鉴定结直肠癌的差异表达基因,并研究候选基因在结直肠癌中表达情况以及其病理特征和预后意义。方法通过分析来自GEO数据库的基因表达数据集来鉴定结直肠癌中的差异表达基因,构建蛋白互作网络,找到核心蛋白,进行基因集富集分析,明确蛋白作用通路,通过免疫组织化学检测结直肠癌组织及正常组织中核心蛋白表达水平,生存分析进一步明确核心基因与结直肠癌的预后关系。结果通过筛选标准鉴定出89个差异表达基因,构建蛋白互相作用网络筛选出核心基因SERPINE1,基因富集分析表明SERPINE1表达与炎症反应及血管生成有关。SERPINE1的高表达与结直肠癌的浸润深度(P=0.012),淋巴结转移(P=0.025),远处转移(P=0.040)和TNM分期(P=0.016)显著相关。生存分析显示SERPINE1的高表达与结直肠癌的整体生存期较差有关(P=0.002)。结论SERPINE1可能是结直肠癌发展过程中的致癌基因,SERPINE1高表达是结直肠癌预后不良的因素。

Objective To identify differentially expressed genes in colorectal cancer through bioinformatics,and to study the expression of candidate genes in colorectal cancer,as well as their pathological characteristics and prognostic significance.Methods Differentially expressed genes in colorectal cancer were identified by analyzing gene expression data sets from the GEO database,protein interaction networks was constructed,core proteins were found,gene set enrichment analysis was performed,and protein action pathways were clarified,then core protein expression levels between colorectal cancer tissues and normal tissues was compared by immunohistochemistry,finally the relationship between core genes and the prognosis of colorectal cancer was further clarified though survival analysis.Results A total of 89 differentially expressed genes were identified by screening criteria,and the core gene SERPINE1 was screened though constructing protein interaction network.Gene enrichment analysis showed that SERPINE1 expression was related to inflammation and angiogenesis.The high expression of SERPINE1 was significantly related to the invasion depth(P=0.012),lymph node metastasis(P=0.025),distant metastasis(P=0.040),and TNM stage(P=0.016)of colorectal cancer.Survival analysis showed that high expression of SERPINE1 was associated with poor overall survival of colorectal cancer(P=0.002).Conclusion SERPINE1 may be an oncogene in the development of colorectal cancer,and high expression of SERPINE1 is a poor prognostic factor for colorectal cancer.