采用MiSeq测序技术分析3种飞虱中肠内容物的菌群结构

Diversities of microbial communities in midgut of 3 planthoppers species by MiSeq

运用MiSeq测序技术比较了褐飞虱(Nilaparvata lugens St l,BPH)、灰飞虱(Laodelphax striatella Fallén,SBPH)和白背飞虱(Sogatella furcifera Horvath,WBPH)体内中肠细菌群落结构的异同。结果显示:3种飞虱共获得可用于分析的有效序列3.44×10^5条,褐飞虱、灰飞虱和白背飞虱在3%水平和6%水平分别获得76、103、61和49、68、42个操作分类单元(operational taxonomic unit,OTU)。灰飞虱体内约90%的中肠细菌属于F-proteobacteria-unclassified;Arsenophonus是褐飞虱体内的优势菌,约占总量的70%;在白背飞虱中Halomonas、Candidatus_Cardinium和Arsenophonus三种菌含量都比较高,分别占35%、20%和15%。虽然3种飞虱从共同的祖先分化,但其中肠内细菌微生物群落多样性、丰富度仍存在一定程度的差异。

In this study,we successfully demonstrated that MiSeq was a powerful approach for investigating the bacterial communities in midgut of brown planthopper(BPH)Nilaparvata lugens St l,white-backed planthopper(WBPH)Sogatella furcifera Horvath and small brown planthopper(SBPH)Laodelphax striatellus(Fallen).For all three planthoppers,3.44×10^5 effective sequences were selected and utilized for the bacterial diversity and abundance analysis.In total,76,103,and 61 operational taxonomic units were obtained at 3%distance cutoff,49,68 and 42 at 6%distance cutoff in the BPH,SBPH and WBPH,respectively.Nearly 90%of the sequences in the SBPH were assigned into F-proteobacteria-unclassified.In the other two samples,Arsenophonus was the dominant genus in BPH,accounting for about about 70%sequences,while in WBPH,Halomonas,Candidatus_Cardinium and Arsenophonus were 3 dominant bacterias,accounting for about 35%,20%and 15%,respectively.Though they were evolved from the common ancestor,the microbial community diversity,richness,dominant bacteria function of endosymbiont in three planthoppers were different.