基于TLR4/NF-κB信号通路探讨地胆头干预社区获得性肺炎机制研究及临床观察

Effect of Elephantopus scaber Linn on community acquired pneumonia through TLR4/NF-κB signaling path signaling pathway

目的:观察地胆头对社区获得性肺炎的疗效,探讨其与TLR4/NF-κB信号转导通路和下游炎性因子IL-6、IL-8、TNF-α表达的关系,以期为社区获得性肺炎的治疗提供新的思路和理论依据。方法:将140例社区获得性肺炎患者,随机分为观察组及对照组各70例,观察组:地胆头液+生理盐水雾化吸入+头孢他啶针静脉注射;对照组:头孢他啶针静脉注射治疗,选择同期70名健康体检者为健康对照组;用酶联免疫吸附法(ELISA)检测观察组、对照组治疗前后及健康对照组血清中TLR4、NF-κB、IL-6、IL-8、TNF-α表达,并对照观察地胆头治疗社区获得性肺炎的效果。结果:与对照组相比,观察组痊愈率、总有效率、发热、咳嗽、咳痰症状消失天数明显优于对照组,有统计学意义(P<0.05);与健康对照相比,观察组及对照组血清TLR4、NF-κB、IL-6、IL-8、TNF-α有明显差异(P<0.05);地胆头治疗前后,观察组血清TLR4、NF-κB、IL-6、IL-8、TNF-α有明显差异(P<0.05);与对照组治疗后比,观察组治疗后血清TLR4、NF-κB、IL-6、IL-8、TNF-α有明显差异(P<0.05)。结论:社区获得性肺炎患者血清TLR4、NF-κB、IL-6、IL-8、TNF-α呈高表达状态,地胆头液降低血液中TLR4、NF-κB的表达,其下游炎症因子也明显下降,观察组、对照组治疗后血液中TLR4、NF-κB存在明显差异(P<0.05),说明地胆头可能是通过抑制TLR4/NF-κB信号通路来发挥抗炎作用;地胆头治疗社区获得性肺炎疗效确切,能显著改善临床症状,有效缩短治疗周期。

Objective To observe the effect of Elephantopus scaber Linn on community-acquired pneumonia and its relationship with the expression of TLR4/NF-κB pathway and the downstream inflammatory cytokines IL-6,IL-8,TNF-α.MethodsA total of 140 patients with community-acquired pneumonia were randomly divided into observation group and control group,with 70 cases in each group.Patients in observation group were treated by Elephantopus scaber Linn+atomized inhalation of normal saline+intravenous injection of ceftazidine;while patients in control group were treated by intravenous injection of ceftazidine;Meanwhile,70 healthy subjects were selected as the healthy control group.The expressions of TLR4,NF-kB,IL-6,IL-8,and TNF-αbefore and after treatment were detected by enzyme linked immunosorbent assay.ResultsCompared with the control group,recovery rate,total effective rate,disappearance time of symptoms of the observation group was significantly better than the control group(P<0.05).Compared with the healthy control group,the serum TLR4,NF-κB,IL-6,IL-8,TNF-αwere significantly higher(P<0.05)in the observation group,which were significantly decreased after treatment with Elephantopus scaber Linn(P<0.05).ConclusionElephantopus scaber Linn can decrease the expression of TLR4 and NF-κB in blood and its downstream inflammatory factor.Elephantopus scaber Linn may inhibit TLR4/NF-kB signal pathway to exert anti-inflammatory effect and can be used to treat community-acquired pneumonia.