miR-186在慢性阻塞性肺疾病患者血清外泌体中表达与临床意义

Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease

目的:研究miR-186在慢性阻塞性肺疾病患者血清外泌体中的表达,探讨其与慢性阻塞性肺疾病(COPD)的临床意义。方法:收集本院53例COPD患者与53例正常人对照外周血血清,抽提外泌体并进行电镜鉴定外泌体特征,RT-PCR方法检测血清外泌体中miR-186的表达量并统计分析COPD组与正常组表达差异,分析miR-186与FEV1%预计值相关性,以及ROC曲线分析血清外泌体miR-186在COPD的诊断价值。结果:透射电镜观察到血清外泌体直径约100 nm的球形结构形态;COPD组血清外泌体中miR-186相对表达量明显高于正常对照组(t=15.47,P<0.001);miR-186表达水平与COPD患者的FEV1%预计值呈负相关(r=-0.5024,P<0.0001);miR-186区分COPD患者与正常人的ROC曲线下面积为0.8409(95%置信区间:0.6875~0.9943)。结论:血清外泌体中miR-186高表达可能是危险因素,可能作为COPD的诊断指标。

Objective To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease,and to explore its clinical significance with chronic obstructive pulmonary disease.Methods The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected.Extraction of exosomes and electron microscopy to identify the characteristics of exosomes.RT-PCR method was used to detect the miR-186 expression level in serum exosomes.And statistical analysis was conducted to compare the expression difference between the COPD group and the normal group,the correlation between miR-186 and FEV1%predicted value,and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD.Results The relative expression of miR-186 in serum exosomes of COPD group was 3.24±0.14,which was significantly higher than that of normal control group 1.03±0.04(t=15.47,P<0.001).The expression level of miR-186 was negatively correlated with the predicted FEV1%of COPD patients(r=-0.5024,P<0.0001).The area under the ROC curve between miR-186 and COPD patients was 0.8409(95%CI 0.6875-0.9943).Conclusions The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD.