通腑理肺汤对脓毒症肠屏障损伤肠组织Claudin-1 mRNA及蛋白表达的影响

The effect of tong-fu-li-fei decoction on the expression of claudin-1 mRNA and protein in intestinal tissue of sepsis rats with intestinal barrier injury

目的探讨通腑理肺汤(TFL)对脓毒症大鼠肠组织Claudin-1 mRNA及蛋白表达的影响。方法 SD大鼠按随机数字表法分为假手术组、模型组、3.6g/kg TFL治疗组、7.2g/kg TFL治疗组,除假手术组只暴露盲肠外,其余各组采用盲肠结扎穿孔(CLP)法制作脓毒症大鼠模型。3.6g/kg TFL治疗组、7.2g/kg TFL治疗组通过灌服接受通腑理肺汤煎剂,剂量分别为3.6及7.2g/kg体重,假手术组和模型组每天通过灌服给予蒸馏水(10ml/kg),7天后处死大鼠,剥离其肠组织,采用实时定量PCR(RT-qPCR)法检测肠组织紧密连接Claudin-1 mRNA基因,Western Blot检测Claudin-1蛋白表达。结果与假手术组相比,模型组大鼠肠组织中Claudin-1 mRNA基因及蛋白表达均显著下降(P<0.05)。与模型组相比,给药组大鼠肠组织中Claudin-1 mRNA、蛋白表达水平显著上升(P<0.05)。结论通腑理肺汤可通过上调脓毒症模型大鼠肠组织紧密连接蛋白Claudin-1 mRNA及蛋白的表达,改善肠上皮细胞间的紧密连接作用,修复脓毒症造成的肠屏障损伤。

Objective To investigate the effect of tong-fu-li-fei decoction(TFL)on the expression of claudin-1 mRNA and protein in the intestinal tissue of sepsis rats.Methods The SD rats were divided into the sham group,model group,the TFL 3.6 g/kg and 7.2 g/kg-treated group.The sepsis model was induced by cecal ligation and puncture(CLP)except the sham group which only exposed cecum.TFL 3.6g/kg treatment group and TFL 7.2g/kg treatment group received tong-fu-li-fei decoction by gavage at doses of 3.6g/kg and 7.2g/kg body weight respectively,distilled water(10ml/kg)was given daily in sham group and model group,the rats were killed after 7 days and their intestinal tissues were removed,the real time quantitative PCR(RT-qPCR)was used to detect thetight junction complex claudin-1 mRNA in intestinal tissue,Western blotting analysis was used to detect protein expression of claudin-1.Results Compared with the sham group,the expression of claudin-1 mRNA and protein in the intestinal tissue of the model group decreased significantly(P<0.05);Compared with the model group,the expression level of claudin-1 mRNA and protein in intestinal tissue of rats in the administration group increased significantly(P<0.05).Conclusion Our data showed that tong-fu-li-fei decoction can upregulate the expression of claudin-1 mRNA and protein in intestinal tissue of sepsis model rats,improve the tight connection complex between intestinal epithelial cells and repair the intestinal barrier injury caused by sepsis.