野生絮缘蘑菇ITS鉴定及其生物学特性

ITS identification and biological characteristics of Agaricus subfloccosus

【目的】对采集于祁连山排露沟流域海拔2900 m青海云杉林地中的一株野生蘑菇进行分类鉴定和生物学特性研究,为其开发利用提供参考.【方法】提取野生蘑菇分离纯化后的菌丝体DNA进行rDNA-ITS扩增,利用BLAST比对和系统进化树构建鉴定蘑菇种类;配制不同碳源、不同氮源、不同pH值培养基,设置不同培养温度,观测不同培养下的菌丝长速、菌丝长势、菌丝形态3指标.【结果】由野生蘑菇分离纯化得到的菌株为絮缘蘑菇,其菌丝生长的最适碳源为果糖、最适氮源为酵母膏、最适pH值为5.0、最适温度为25℃.【结论】研究结果为野生絮缘蘑菇开发利用提供了参考.

【Objective】The taxonomic identification and biological characteristics of a wild mushroom were studied,which was isolated from Picea crassifolia forest at an altitude of 2900 m in the Pailugou watershed of Qilian Mountains.【Method】The isolated and purified mycelium DNA of the wild mushroom was extracted for amplification of rDNA-ITS.Subsequently,BLAST alignment and phylogenetic tree construction were used to identify the wild mushroom species.Culture medium with different carbon,nitrogen source and different pH value was prepared,observed the growth rate,the growth vigo and morphology of mycelial under different culture temperatures and different culture conditions.【Result】The wild mushroom was confirmed to be Agaricus subfloccosus,which suitable carbon sources for the mycelium growth was fructose,the suitable nitrogen sources was yeast extract,the suitable pH was 5.0,the suitable temperature was 25℃.【Conclusion】The results apply a reference for the utilization of Agaricus subfloccosus.