盐胁迫下巴西蕉叶片的转录组和蛋白质组关联分析

Association Analysis on Transcriptomics and Proteomics of Musa paradisiaca Banana Leaf under Salt Stress

为了从转录组和蛋白质组水平上揭示巴西蕉响应盐胁迫环境的分子机制,本研究以中国主栽品种‘巴西蕉’(Musa paradisiaca)为试验材料,人工模拟盐胁迫60 mmol/L NaCl分别处理0 h、12 h和24 h,利用RNA-Seq和iTRAQ技术对叶片进行转录组和蛋白质组关联分析。本研究共鉴定出68个与差异基因表达趋势相同的差异蛋白,其中13个上调,55个下调。这些差异蛋白功能涉及细胞骨架、结构、抗氧化物、胁迫防御、代谢和其它未知功能蛋白等。上调表达的蛋白主要参与了细胞骨架、结构和胁迫防御等,包括微管蛋白、凝集素和β-半乳糖苷酶等;下调表达的蛋白主要参与抗氧化物和代谢等,主要包括放氧增强蛋白、半胱氨酸合酶、蔗糖合酶等。通过高通量多组学数据的关联分析为深入认识巴西蕉响应盐胁迫的分子调控机制提供了理论依据。

In order to reveal the molecular mechanism of Musa paradisiaca banana response to salt stress at gene and protein level by RNA-Seq and iTRAQ technology and Musa paradisiaca was used as materials,the 60 mmol/L NaCl leaves under artificial simulated salt stress for 0,12 and 24 hours were recorded and association analysis on transcriptomics and proteomics was carried out,and 68 distinct proteins with the same expression tendency of differential genes were identified among them,13 were up-regulated and 55 were down-regulated.These functions of distinct proteins involved cytoskeleton,structure,antioxidants,stress defense,metabolism and other unknown functional proteins.The up-regulated proteins are mainly involved in cytoskeleton,structure,and stress defense,including tubulin,lectin,β-galactosidase,etc.And the down regulated proteins are mainly involved in antioxidants and metabolism,including oxygen releasing enhancement protein cysteine synthetase sucrose synthetase,etc.The association analysis of high-throughput and multi-component data laid a foundation for further understanding the molecular regulation mechanism of banana response to salt stress.