木本植物瞬时转化体系的研究进展

Advances in Transient Transformation Systems of Woody Plants

木本植物是经济树种与观赏植物的重要组成部分,其快繁、改良、育种一直都是研究热点。近年来,功能基因组学以及基因修饰技术的发展更为木本植物的定向培育提供了广阔空间。遗传转化体系是植物基因工程的核心,也是进行植物改良与精确育种的重要途径。稳定的遗传转化对植物自身生长和繁殖特性要求比较严格,且转化时间长,效率低,不能满足当下快速鉴定相关基因功能、了解未知代谢途径以及进行人工调控的需求,而瞬时转化技术作为一种高效便捷的手段,已被越来越多的应用于木本植物相关基因功能研究。本研究主要从瞬时转化方法以及应用情况等方面,论述了近年来木本植物瞬时转化体系的研究进展,为木本植物基因工程育种和种质创新提供参考。

Woody plant plants are an important part of economic and horticultural crops.The rapid propagation,improvement and breeding of economic trees and woody ornamental plants have always been the research focus.In recent years,the rapid development of functional genomics and gene modification technology has provided a broad space for the targeted cultivation of woody plants.Genetic transformation system is the core part of plant genetic engineering,and has been taken an important way in plant improvement and precise breeding.The stable genetic transformation of wooden crop is dependent on the strict growth conditions and the reproduction char-acteristics of plants.The transformation time is usually very long and the transgene efficiency is low,which can not meet the current needs of rapid identification of related gene functions,understanding of unknown metabolic pathways and artificial regulation.As an efficient and convenient means,transient transformation technology has been increasingly applied in the function research of related genes in woody plants.Here,the researth progress of the transient transformation system of woody plants in recent years is reviewed from the aspects of transient transformation methods and application,which could provide helpful guidance for the genetic engineering breeding and germplasm innovation of woody plants.