妊娠期糖尿病患者胎儿脐带基因表达差异研究

Exploring the Gene Changes in the Umbilical Cord of Gestational Diabetes Mellitus Patients

目的:运用高通量测序数据对妊娠期糖尿病(gestational diabetes mellitus,GDM)患者生物信息进行分析,探讨GDM对胎儿基因表达的影响。方法:NCBI网站GEO数据库获取GDM患者胎儿脐带组织高通量测序数据,建立条件筛选差异表达基因(differentially expressed genes,DEGs),使用DAVID在线工具分析DEGs相关的生物过程与生物信号传导通路,使用STING在线工具分析GEGs编码蛋白之间的相互关系(protein protein interaction,PPI),并筛选出候选基因。选取60名GDM患者(实验组)与GDM名正常孕妇(对照组)脐带组织,利用实时荧光定量PCR技术(real-time fluorescent quantitative PCR,qRT-PCR)验证候选基因的表达。结果:共计113个DEGs满足筛选条件,其中低表达基因30个,高表达基因83个。PPI分析确定19个基因为可能的候选基因。经qRT-PCR验证,实验组HLA-DRB5基因表达显著低于对照组,差异有统计学意义(P<0.01),实验组ALDH1A2、SLC4A1、TP63基因显著高于对照组,差异有统计学意义(P<0.01)。结论:GDM患者高糖环境导致胎儿脐带基因表达差异,胎儿生长发育可能受差异表达基因影响,检测GDM患者胎儿脐带基因表达有望为揭示胎儿生长发育异常提供科学依据。

Objective:To explore the bioinformatics and gene changes in the umbilical cord of patients with gestational diabetes mellitus(GDM)based on high throughput sequencing.Methods:High throughput sequencing data was gained from NCBI GEO data sets.DEGs were identified by screening condition.The online DAVID software were performed to examine the biology process and signaling pathway.Protein protein interaction(PPI)analysis was performed using STRING online tool,and hub genes were screened out by STRING analysis.qRT-PCR was carried out to validate the expression level of candidate genes in 60 umbilical cord tissue from patients with GDM(test group)and 60 umbilical cord tissue from healthy pregnant women(control group).Results:A total of 113 DEGs were obtained,including 83 up-regulated genes and 30 down-regulated genes.19 genes were screened out as candidate genes by PPI analysis.By analysis of qRT-PCR,HLA-DRB5 was validated low expression in test group(P<0.01),ALDH1A2,SLC4A1,TP63 were validated high expressions in test group(P<0.01).Conclusion:The high-glucose environment in GDM patients leads to the gene changes in fetal umbilical cord,and fetal development may be affected by GEGs.The detection of fetal umbilical cord gene expression in GDM patients is expected to provide scientific basis for revealing fetal growth and development abnormalities.