2015—2019年上海等地区质谱细菌鉴定室间质量评价结果分析

External quality assessment for bacterium identification by mass spectrometry from 2015 to 2019 in Shanghai and several regions

目的通过开展质谱细菌鉴定室间质量评价(简称室间质评)活动,了解上海及部分其他地区医疗机构质谱细菌鉴定的检测质量。方法收集2015—2019年参加上海市临床检验中心(SCCL)质谱细菌鉴定室间质评项目的反馈结果,对细菌鉴定符合率进行统计分析。结果参加SCCL开展的质谱细菌鉴定室间质评活动的实验室由2015年的10家增加到2019年的40家,5年室间质评的合格率均为100.0%。发放的50株菌株(涵盖27个属43个种)中,有45株鉴定结果符合率为100%,鉴定结果有误的菌株有维罗纳气单胞菌、温和气单胞菌、中间葡萄球菌、表皮葡萄球菌、肺炎链球菌,符合率分别为60.0%、94.4%、95.5%、89.7%、92.5%。结论上海及部分其他地区开展质谱细菌鉴定的医疗机构逐年增多,主要为三级医院,总体检测能力和准确率较高,但个别实验室检测能力有待提高。临床实验室的质量控制对于保证检测结果的准确性具有十分重要的作用。

Objective To study the quality of mass spectrometry(MS)bacterium identification of medical laboratories in Shanghai and several regions.Methods The feedback results of MS bacterium identification external quality assessment in Shanghai Center for Clinical Laboratory(SCCL)from 2015 to 2017 were collected,and the consistency of bacterium identification was analyzed.Results The number of laboratories participating SCCL microbial MS bacterium identification external quality assessment increased from 10 in 2015 to 40 in 2019.The qualification rate remained 100.0%during the 5 years.A total of 50 isolates of bacteria were delivered(covering 27 genera and 43 species).The consistency rate was 100.0%.The isolates with incorrect identification results included Aeromonas veronii,Aeromonas sobria,Staphylococcus intermedius,Staphylococcus epidermidis and Streptococcus pneumoniae,and the consistency rates were 60%,94.4%,95.5%,89.7%and 92.5%,respectively.Conclusions The number of laboratories,mainly in Grade 3 hospitals,setting MS bacterium identification in Shanghai and several regions,increases year by year.The overall detection ability and accuracy are good and high,while some laboratories'performance still should be improved.Quality control in clinical laboratories is essential to assure accuracy.