响应面法超声辅助优化巨大口蘑多酚的提取工艺及其体外抗氧化活性研究

Antioxidant activities and ultrasound-assisted extraction technique optimization of polyphenols from TrichoLoma giganteum by response surface methodology

以巨大口蘑为研究对象,采用超声波辅助乙醇提取法,以乙醇浓度、料液比、提取时间及提取次数为单因素进行实验,在此基础上,以提取率为响应值,利用Box-Behnken方法优化多酚提取条件,并对其体外抗氧化活性进行研究。结果表明,巨大口蘑多酚提取工艺的最佳条件为:乙醇浓度为39%、液料比21∶1(mL·g^-1)、提取时间40min、提取次数2次,该条件下巨大口蘑中多酚的含量为(9.4072±0.013)mg·g^-1,巨大口蘑多酚的自由基清除能力与多酚浓度呈现出很好的剂量相关性,其中清除DPPH自由基的IC50值为0.032 mg·mL^-1、清除ABTS自由基的IC50值为0.063 mg·mL^-1,在质量浓度为0.1mg·mL^-1时,巨大口蘑多酚的铁离子还原能力为0.364。本研究将为巨大口蘑进一步的开发利用提供重要参考依据。

The extraction technology of polyphenols from TrichoLoma giganteum was optimized and its antioxidant activity was evaluated.The single-factor experiment was carried out by ultrasonic assisted extraction method with ethanol concentration,liquid-solid ratio,ultrasonic time and extraction times.Then the extraction conditions of polyphenols were optimized by the response surface methodology.The antioxidant activity was also evaluated by three methods containing scavenging DPPH free radical,scavenging ABTS free radical and iron reduction capacity.The results showed that the optimal polyphenols extraction conditions were ethanol concentration 39%,liquid-solid ratio 21∶1 mL·g^-1,extraction time 40 min,and extraction times 2 times.Under such an optimization condition,the experimentally measured yield of polyphenols was 9.4072±0.013 mg·g^-1.Besides,the free radical scavenging ability of polyphenol showed a good dose correlation with polyphenol concentration.The IC50 value of scavenging DPPH free radical was 0.032 mg·mL^-1,and that of scavenging ABTS free radical was 0.063 mg·mL^-1.When the mass concentration was 0.1 mg·mL^-1,the iron reduction capacity was 0.364.The study will provide important reference for the further utilization of TrichoLoma giganteum.