基于酶辅助信号放大的纳米石墨传感器检测银离子的研究

Research on Nano-graphite Sensor for Silver Ion Detection Based on Enzyme-Assisted Signal Amplification

利用纳米石墨和单链寡核苷酸组装了一种新型荧光生物传感器,结合脱氧核糖核酸酶作信号放大,实现了对溶液中的银离子高灵敏高选择性的检测。对缓冲液pH和脱氧核糖核酸酶用量等实验条件进行了优化,在最佳实验条件下该传感器对银离子的检出限为0.3 nM,且具有优异的选择性和较好重现性。在实际水样中该方法对银离子检测的回收率为94.0%~97.3%,说明这种传感器可以应用于实际水样中对银离子的检测。

A new type of fluorescent biosensor was assembled using nano-graphite and single-stranded oligonucleotides,combined with deoxyribonuclease for signal amplification,to achieve highly sensitive and selective detection of silver ions in solution.The experimental conditions such as buffer pH and the amount of deoxyribonuclease were optimized.Under the optimal experimental conditions,the detection limit of the sensor for silver ions is 0.3 nM,and it has excellent selectivity and good reproducibility.In the actual water sample,the recovery rate of silver ion detection by this method is 94.0%~97.3%,indicating the sensor can be applied to the detection of silver ions in actual water samples.