miR-365抑制TGF-β诱导的人肺癌细胞上皮-间质转化

miR-365 Inhibits TGF-β Induced Epithelial Mesenchymal Transition in Human Lung Cancer Cells

目的研究miR-365在TGF-β诱导的人肺癌细胞A549上皮-间质转化过程中的作用。方法 TGF-β刺激A549细胞,观察细胞形态变化, RT-PCR检测细胞内miR-365的表达;通过感染慢病毒,使A549细胞中miR-365过表达,Western blot检测上皮-间质转化相关蛋白E-cadherin的表达。结果 TGF-β刺激A549细胞后,细胞形态发生改变,呈长纤维状,胞内miR-365表达上升;过表达miR-365,E-cadherin表达升高。结论 miR-365可抑制TGF-β诱导的人肺癌细胞上皮-间质转化。

Objective To study the effects of miR-365 on the epithelial mesenchymal transition(EMT)in human lung cancer cell A549 induced by TGF-β.Methods After stimulating A549 cells with TGF-β,the changes of cell morphology were observed,and the expression of miR-365 in the cells was detected by RT-PCR.Through infection with lentivirus,miR-365 was overexpressed in A549 cells,and Western blot was used to detect the expression of epithelial mesenchymal trans ition related protein E-cadherin.Results After TGF-βstimulated A549 cells,the cell morphology changed,showing a long fibrous shape,and the expression of miR-365 in the cell increased.Overexpression of mir-365 increased the expression of E-cadherin.Conclusion miR-365 can inhibits epithelial mesenchymal transformation in human lung cancer cells induced by TGF-β.