山药低聚糖制备分离及对五种益生菌的增殖作用

Preparation and isolation of yam oligosaccharides and its proliferation effect on five probiotics strains

为探究山药低聚糖及其组分分别对不同益生菌的体外增殖作用,利用超声辅助提取法从山药中提取山药低聚糖,并依次通过活性炭色谱柱和Sephadex LH-20凝胶色谱柱,得到了数均分子质量分别为0.74、0.94、1.25 kDa的3个组分山药低聚糖。同时以山药低聚糖作为碳源,增殖培养婴儿双歧杆菌、两歧双歧杆菌、青春双歧杆菌、BB-12双歧杆菌以及罗伊氏乳杆菌5种益生菌。结果表明,相比于低聚木糖培养基,BB-12双歧杆菌、两歧双歧杆菌和罗伊氏乳杆菌的OD 600值分别提高了13.9%,8.24%和7.18%。以分离纯化后的山药低聚糖的3个组分分别作为碳源,增殖培养上述3种益生菌。结果表明,不同组分的山药低聚糖对不同益生菌的增殖作用具有显著性差异。山药低聚糖是一种较好的益生元,具有应用于益生菌业的潜力。

In order to investigate the effects of yam oligosaccharides and their components on in vitro proliferation of different probiotics,ultrasonic extraction method was adopted to extract yam oligosaccharides,which were sequentially passed through activated carbon column chromatography and Sephadex LH-20 gel column chromatography.Three components of yam oligosaccharides were then obtained,with the average molecular weights of 0.74 kDa,0.94 kDa,and 1.25 kDa.At the same time,yam oligosaccharides were used as a carbon source to proliferate and cultivate five probiotic strains:Bifidobacterium infantis,Bifidobacterium bifidum,Bifidobacterium adolescentis,Bifidobacterium animals ssp.lactis BB-12 and Lactobacillus reuteri.Compared with xylo-oligosaccharide medium,the OD 600 value of B.animals ssp.lactis BB-12,B.bifidum and L.reuteri increased by 13.9%,8.24%and 7.18%,respectively.Moreover,using the three components of the isolated yam oligosaccharides as carbon source,the above three probiotics were multiplied and cultured.Results showed that the yam oligosaccharides with different components had significant differences in the proliferation of different probiotics.Therefore,yam oligosaccharide is a good prebiotic and has the potential to be used in the probiotic industry.