榛蘑粗多糖对脂多糖诱导的大鼠急性肺损伤的保护作用

Protective effects of Armillaria mellea polysaccharides on acute lung injury induced by lipopolysaccharide in rats

该文研究榛蘑粗多糖(Armillaria mellea polysaccharides)对脂多糖(lipopolysaccharide,LPS)诱导的大鼠急性肺损伤的保护作用。将35只SD大鼠随机分成5组,分别为正常对照组、模型组(LPS 20 mg/kg)、阳性对照组(地塞米松5 mg/kg)、榛蘑多糖低、高剂量(200、400 mg/kg)组。采用苏木素-伊红染色观察肺组织病理学变化,酶联免疫吸附测定法测定血清内皮素-1(endothelin-1,ET-1)、血栓调节蛋白(thrombomodulin,TM)、白介素-6(interleukin-6,IL-6)以及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平,Western blot法观察I-κB蛋白的表达和PI3K、Akt以及NF-κB p65蛋白的磷酸化水平。结果表明,与模型组相比,榛蘑粗多糖组大鼠肺泡结构破坏减轻,肺泡壁增厚减轻,无微血栓形成。并且,血清TNF-α、IL-6、ET-1以及TM的含量明显减少。同时,p-PI3K、p-Akt和p-NF-κB p65水平明显降低,而I-κB表达明显增多。说明榛蘑粗多糖对LPS诱导的大鼠急性肺损伤具有保护作用,其作用机制可能与它对PI3K/Akt信号通路的调控有关。

To investigate the protective effect of Armillaria mellea polysaccharides on acute lung injury induced by lipopolysaccharide (LPS) in rats, thirty-five healthy SD rats were randomly divided into five groups: The normal control, model (LPS 20 mg/kg), positive control (dexamethasone 5 mg/kg ), low (200 mg/kg) and high (400 mg/kg) dose groups of Armillaria mellea polysaccharides. The pathological changes on lung tissue were tested by HE staining, the levels of ET-1, TM, IL-6 and TNF-α in serum were measured by ELISA, the expression of I-κB protein and the phosphorylation of PI3K, Akt and NF-κB p65 was observed with the Western blot method. The results showed that compared with the model group, the thickening of alveolar wall was reduced, and no thrombus was observed in the group of Armillaria mellea polysaccharides. In addition, the levels of TNF-α, IL-6, ET-1 and TM were significantly reduced. At the same time, the phosphorylations of PI3K, Akt and NF-κB p65 were significantly decreased and the expression of I-κB was significantly increased. These results indicated that the Armillaria mellea polysaccharides had the protective role in acute lung injury induced by LPS in rats. The mechanism of the action may be related to its regulation of the PI3K/Akt signaling pathway.