排水管道沉积物微生物群落及环境因子分析

Analysis of microbial communities and environmental factors in sewer sediments

选取北京市某区的排水管道沉积物进行取样,采用高通量测序手段分析,结果表明变形菌门、广古菌门、拟杆菌门、厚壁菌门是排水管道沉积物微生物中的优势门类;在纲水平上,δ-变形菌纲、甲烷微菌纲、梭菌纲、拟杆菌纲占相对优势;在属水平上,功能性微生物硫酸盐还原菌(SRB)和产甲烷古菌(MA)普遍存在于各处管道.在所选的6段管段中,管段S3、S4处MA的相对丰度分别为20.6%、40.8%,高于其他管段,厌氧产甲烷的潜能较大,有发生可燃气积累的风险;管段S5、S6处的SRB相对丰度分别为9.14%、8.19%,高于其他管段,硫酸盐还原为硫化物的潜能较大,存在管道腐蚀的风险.RDA分析表明污水的DO、水温、硫酸根、TN与管道沉积物中微生物群落存在相关性.

Sewer sediments were sampled in a district of Beijing and analyzed using high-throughput sequencing,it was found that Proteobacteria,Euryarchaeota,Firmicutes,Bacteroidetes were the dominant phyla,and Deltaproteobacteria,Methanomicrobia,Clostridia,Bacteroidia were the dominant classes of all samples.The functional microorganisms Sulfate-Reducing Bacteria(SRB)and Methanogenic Archaea(MA)were prevalent in sewer sediments at the genus level.Among the six sewer pipes sampled,the relative abundances of MA were 20.6%and 40.8%in the sewer pipes S3and S4,higher than other sewer pipes,which implied higher the methane production potential.Thus there was a risk of combustible gas accumulation in S3and S4.The relative abundances of SRB were 9.14%and 8.19%in the sewer pipes S5and S6,higher than other sewer pipes,which implied that the potential of sulfate reduced to sulfide was greater.Thus there was a risk of sewer corrosion in S5and S6.Redundancy Analysis(RDA)denoted that the dissolved oxygen,temperature,sulfate,total nitrogen of sewage had correlation with the microbial communities in the sewer pipe sediments.