明月草组培快繁技术体系的建立

Establishment of tissue culture and rapid propagation technology system of Angelica keiskei Koidzumi

以明月草(Angelica keiskei Koidzumi)茎段为外植体进行组培快繁技术研究,通过设置不同处理探究了外植体消毒、腋芽诱导、增殖培养、生根培养、炼苗移栽5个环节最佳处理方式和培养基配方,建立了明月草的组培快繁技术体系。结果表明,使用0.1%HgCl2消毒10 min可以达到理想消毒效果,污染率控制在13.3%,萌芽率可达到83.3%,有效芽率达到96.0%。明月草芽诱导最优培养基为MS+0.80 mg/L 6-BA+0.10 mg/L NAA,培养4~6 d芽开始萌动;最适宜用做明月草继代增殖的培养基为MS+0.80 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L AD,增殖系数为3.39;单一使用IBA或NAA的生根效果优于二者组合,当NAA浓度为0.40 mg/L时,培养10 d,生根率可达到100%,且生根数及平均根长均优于其他处理组合;选用黄心土作为移栽基质,移栽根系完整的明月草组培苗,20 d后调查成活率可达98%以上。

Taking the Angelica keiskei Koidzumi as test materials,through setting up different treatments,the optimal treatment meth⁃ods and medium formulations of explants disinfection,axillary bud induction,proliferation culture,rooting culture and seedling trans⁃planting were studied,and the tissue culture and rapid propagation technology system of the plant was established.The results showed that using 0.1%HgCl2 disinfection for 10 min can achieved the ideal disinfection effect,the contamination rate was controlled at 13.3%,the germination rate was up to 83.3%,and the effective buds were up to 96.0%.The optimal culture medium for bud induction was:MS+0.80 mg/L 6-BA+0.10 mg/L NAA,and the buds began to germinate in about 4~6 days.The most suitable culture medium for subculture was MS+0.80 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L AD,and the coefficient of proliferation was 3.39.The rooting ef⁃fect of single use of IBA or NAA is better than the combination of the two.When NAA concentration is 0.40 mg/L,the rooting rate reached 100%when cultured for 10 days,and the rooting number and average root length are better than other treatment combinations.The yellow-soil was selected as the transplanting substrate,and the seedlings with complete root system were transplanted.The surviv⁃al rate was over 98%after 20 days.