摘要
建立了复方丹皮酚纳米乳中丹皮酚和灵芝多糖的含量测定方法,丹皮酚测定选用HPLC法,灵芝多糖的测定采用蒽酮硫酸比色法。结果3批乳剂中丹皮酚、灵芝多糖的平均含量分别为7.612和6.127 mg/mL,平均包封率为97.99%,含量稳定均一,包封率高。该方法简便、准确可靠、重复性好,可作为复方丹皮酚纳米乳中丹皮酚和灵芝多糖的含量测定方法。
Establish a method to determine the content of paeonol and ganoderan in Compound Paeonol Nanoemulsion.The paeonol content was determined using HPLC.Determination of ganoderan was performed using the anthrone-sulfuric acid colorimetric method.The average contents of paeonol and ganoderan in the three nanoemulsions were 7.612 and 6.127 mg/mL,respectively.The average encapsulation efficiency was 97.99%.The contents were stable and uniform,and the encapsulation efficiency was high.This method is simple,reproducible,accurate,and reliable.It can be used to determine paeonol and ganoderan content in Compound Paeonol Nanoemulsion.
作者
苏酩
袁敏
孙晓彤
隋在云
SU Ming;YUAN Min;SUN Xiao-tong;SUI Zai-yun(Shandong Academy of Traditional Chinese Medicine,Jinan 250014, China;Weifang Hongjing Traditional Chinese Medicine School,Weifang 261057,China)
出处
《山东科学》
CAS
2020年第6期27-33,共7页
Shandong Science
基金
山东省重点研发项目(2015GSF119030,2018GSF119001,2018GSF119023)。
关键词
复方丹皮酚纳米乳
丹皮酚
灵芝多糖
包封率
Compound Paeonol Nanoemulsion
paeonol
ganoderan
encapsulation efficiency
