花粉的表面增强拉曼光谱鉴别研究

Discrimination of Pollen by Surface Enhanced Raman Spectroscopy

为区分不同种类花粉,采用扫描电子显微镜(Scanning Electron Microscope,SEM)和表面增强拉曼光谱对雪松花粉和茶花蜂花粉、荷花蜂花粉、玫瑰蜂花粉、荞麦蜂花粉、五味子蜂花粉、益母草蜂花粉、油菜蜂花粉等七种蜂花粉进行形貌观察和光谱表征。结果显示:花粉间的形态差异微小,扫描电子显微镜难于区分各种花粉,而表面增强拉曼光谱可以简单快速的鉴别不同种类花粉。七种蜂花粉在1800~400 cm^-1范围存在明显差异。相比蜂花粉,雪松花粉的拉曼信号更清晰可辨,其主要特征谱带出现在1701、1657、1572、1522、1397、1293、1208、810和565 cm^-1附近。

In order to distinguish different kinds of pollen,Pinus massoniana Lamb pollen and seven kinds of bee pollen(camellia pollen,lotus pollen,rose pollen,buckwheat pollen,schisandra pollen,motherwort pollen and rape pollen)were observed by scanning electron microscope,and were characterized by surface enhanced Raman spectroscopy.The results showed that the morphological differences among pollens were small,and it was difficult to distinguish different kinds of pollens by scanning electron microscopy,while SERS could identify different kinds of pollen simply and quickly.Seven kinds of bee pollen had significant difference in the range of 1800-400 cm^-1.Compared with bee pollens,Pinus massoniana Lamb pollen had more clearly discernible Raman peaks,and its main characteristic peaks appear near 1701、1657、1572、1522、1397、1293、1208、810 and 565 cm^-1.