免疫组织化学法双色银染原位杂交与荧光原位杂交技术检测乳腺癌HER-2基因状态的应用

The Application of Immuno-Histochemistry,Dual-Color Silver-Enhanced Insitu Hybridization and Fluorescence Insitu Hybridization to Detect the HER-2 Gene Status in Breast Cancer

目的:比较免疫组织化学法(IHC)、双色银染原位杂交(DISH)、荧光原位杂交技术(FISH)对乳腺癌HER-2基因状态的应用效果。方法:回顾性的选取2017年1月至2019年12月我院收治的180例乳腺癌患者作为研究对象,均为女性、单侧,取组织标本行IHC、DISH、FISH检查HER-2。结果:180例患者中各检测方式对HER-2基因的检查效果:IHC检查HER-2表达阴性118例、HER-2表达阳性49例,可疑阳性13例;DISH检查HER-2基因扩增56例、无扩增107例、不确定17例;FISH检查HER-2扩增54例、无扩增107例、不确定19例。各检测方式比较:DISH与IHC检查HER-2基因的Kappa值为0.788,一致性较好;FISH与IHC检查HER-2基因的Kappa值为0.784,一致性较好;DISH与FISH检查HER-2基因的Kappa值为0.939,一致性高。结论:IHC、DISH、FISH均可对乳腺癌患者的HER-2基因状态行评估,可以IHC作为筛查手段,若医疗资源许可应当结合DISH检查结果制定治疗方案。

Objective:To compare the effects of immuno-histochemistry(IHC),dual-color silver-enhanced insitu hybridization(DISH)and fluorescence insitu hybridization(FISH)on the status of breast cancer HER-2 gene.Methods:Retrospective study was conducted on 180 cases of unilateral breast cancer patients admitted to our hospital from January 2017 to December 2019,all of whom were female.Tissue specimens were collected and examined for HER-2 by IHC,DISH and FISH.Results:Results of HER-2 gene in 180 patients:IHC test showed negative expression of HER-2 in 118 patients and positive expression of HER-2in 49 patients.DISH detection of HER-2 gene amplification was performed in 56 cases,107 cases without amplification and 17 cases with uncertainty.FISH detected 54 cases of HER-2 amplification,107 cases without amplification,and 19 cases with uncertainty.Comparison of different detection Methods:the Kappa value of HER-2 gene detected by DISH and IHC was 0.788,showing good consistency;The Kappa value of HER-2 gene examined by FISH and IHC was 0.784,wIHCh was highly consistent;The Kappa value of HER-2 gene detected by DISH and FISH was 0.939,indicating a high consistency.Conclusion:IHC,DISH and FISH can evaluate the her-2 gene status of breast cancer patients,and IHC can be used as a screening method.If medical resources permit,treatment plans should be made based on DISH test results.