急性前葡萄膜炎患者血清IL-35和TGF-β1表达水平及临床意义

Serum IL-35 and TGF-β1 expression levels in patients with acute anterior uveitis and their clinical significance

目的:研究急性前葡萄膜炎患者血清IL-35、TGF-β1表达水平变化及两者之间的相关性,探讨IL-35、TGF-β1在急性前葡萄膜炎中的临床意义。方法:纳入2018-05/2019-05在甘肃省人民医院眼科确诊的急性前葡萄膜炎患者30例作为急性前葡萄膜炎组,同期进行体检的健康人30例作为健康对照组,采用ELISA法检测两组受检者血清IL-35、TGF-β1表达水平,并根据改良的EIU临床评分标准评估急性前葡萄膜炎严重程度。结果:急性前葡萄膜炎患者血清IL-35、TGF-β1表达水平显著高于健康对照组(均P<0.05),且急性前葡萄膜炎患者血清IL-35、TGF-β1水平与疾病严重程度无明显相关性(rs=0.087、0.044,均P>0.05),但血清IL-35与TGF-β1表达水平呈正相关(rs=0.637,P<0.001)。结论:血清IL-35、TGF-β1水平变化与急性前葡萄膜炎的发生和发展密切相关,可能在急性前葡萄膜炎中协同发挥免疫抑制作用。

AIM:To study the changes of serum IL-35 and TGF-β1 expression levels and the correlation between them in patients with acute anterior uveitis,and to explore the clinical significance of IL-35 and TGF-β1 levels in patients with acute anterior uveitis.METHODS:Thirty patients with acute anterior uveitis confirmed in the Department of Ophthalmology of Gansu Provincial Hospital into 2018-05/2019-05 were selected as the case group,and thirty healthy patients who received physical examination at the Gansu Provincial Hospital during the same period were selected as the control group.Serum IL-35 and TGF-β1 expression levels between the two groups were detected by Elisa.Modified endotoxin-induced uveitis(EIU)clinical standard was used for the severity of acute anterior uveitis.RESULTS:Serum IL-35 and TGF-β1 expression levels in the acute anterior uveitis group were significantly higher than that in the healthy control group(all P<0.05),and there was no significant correlation between serum IL-35 and TGF-β1 levels as well as the severity of acute anterior uveitis(rs=0.087,0.044,all P>0.05).There was a significant positive correlation between serum IL-35 and TGF-β1 expression levels in patients with acute anterior uveitis(rs=0.637,P<0.001).CONCLUSION:The expression levels of IL-35 and TGF-β1 in serum are closely related to the occurrence and development of acute anterior uveitis and may play a synergistic role in immunosuppression in acute anterior uveitis.