人高度近视眼房水中免疫因子含量的研究

Levels of immune factors in aqueous humor in high myopia

目的比较高度近视患者及正视眼患者房水中与胶原代谢相关,或与损伤巩膜胶原的眼部炎性疾病相关的免疫因子的表达情况,探讨房水中的免疫因子与高度近视的相关性。设计前瞻性对照研究。研究对象2019年9~12月北京同仁眼科中心高度近视20例(实验组)和正视眼白内障20例(对照组)的房水标本。方法将房水标本通过流式CBA法检测白介素-6(IL-6)、IL-10、转化生长因子-β1(TGF-β1)、碱性成纤维细胞生长因子(bFGF)、IL-1β的含量。比较两组间各因子含量的差异。主要指标房水中IL-6,IL-10,TGF-β1,bFGF,IL-1β的含量。结果实验组和对照组患眼的房水中IL-6分别为(7.60±7.64)pg/ml和(30.02±43.02) pg/ml(t=-2.71,P<0.01);IL-10分别为(1.00±0.91)pg/ml和(1.84±0.97) pg/ml(t=-2.29,P=0.02);bFGF分别为(0.00±0.00)pg/ml和(0.56±1.20)pg/ml(t=-2.25,P=0.03);TGF-β1分别为(6.52±10.75)pg/ml和(4.97±7.25)pg/ml(t=-0.43,P=0.67);IL-1β分别为(2.50±2.14)pg/ml和(2.91±1.12)pg/ml(t=-0.72,P=0.47)。结论高度近视患者房水中免疫因子表达与正视眼者存在差异,可能与高度近视发病原因相关。

Objective To compare the expression of immune factors(IL-6,IL-10,TGF-β1,bFGF,IL-1 β) in aqueous humor of patients with high myopia and emmetropia,to explore the relationship between immune factors in aqueous humor and the incidence of high myopia.Design Prospective control study.Participants Forty aqueous humor was collected from 20 patients with high myopia(experimental group) and 20 patients with emmetropia cataracts(control group).Methods The levels of interleukin-6(IL-6),IL-10,transforming growth factor-β1(TGF-β1),basic fibroblast growth factor(bFGF),and IL-1β in aqueous humor were measured by flow CBA.The different of these factors levels was compared between the two groups.Main Outcome Measures The levels of IL-6,IL-10,TGF-β1,bFGF,and IL-1β in aqueous humor.Results The levels of IL-6 in aqueous humor of experimental group and control group was(7.60±7.64) pg/ml and(30.02±43.02) pg/ml(t=-2.71,P<0.01);the levels of IL-10 was(1.00±0.91) pg/ml and(1.84±0.97) pg/ml(t=-2.29,P=0.02);the levels of bFGF was(0.00±0.00) pg/ml and(0.56±1.20) pg/ml(t=-2.25,P=0.03);the levels of TGF-β1 was(6.52±10.75) pg/ml and(4.97±7.25) pg/ml(t=-0.43,P=0.67);the levels of IL-1β was(2.50±2.14) pg/ml and(2.91±1.12) pg/ml(t=-0.72,P=0.47).Conclusion The different expression of immune factors in aqueous humor may be related to the pathogenesis of high myopia.