高危人乳头状瘤病毒感染宫颈癌患者miR-34a-5p表达及意义

Expression of MicroRNA-34a-5p in Cervical Cancer Patients with High Risk of Human Papillomavirus Infection and Its Clinical Significance

[目的]探讨高危人乳头状瘤病毒(high risk human papillomavirus,HR-HPV)感染宫颈癌患者组织及血清miR-34a-5p的表达以及血清miR-34a-5p对宫颈癌的诊断价值。[方法]采用RTPCR检测70例HR-HPV阳性宫颈癌组织和癌旁组织、100例HR-HPV阳性宫颈癌患者血清、80例HR-HPV阳性宫颈上皮内瘤病变(CIN)患者血清、80例健康体检者血清中miR-34a-5p的表达水平。Pearson法分析组织和血清中miR-34a-5p表达量的相关性,分析miR-34a-5p与宫颈癌临床病理特征的关系,分析血清miR-34a-5p诊断宫颈癌的价值。[结果]宫颈癌组织miR-34a-5p相对表达量明显低于癌旁组织(P<0.05)。宫颈癌患者血清miR-34a-5p相对表达量明显低于CIN患者和正常人(P<0.05),CIN患者血清miR-34a-5p相对表达量明显低于正常人(P<0.05)。癌组织miR-34a-5p相对表达量与淋巴结转移有关(P<0.05)。血清miR-34a-5p相对表达量与淋巴结转移和远处转移有关(P<0.05)。miR-34a-5p在癌组织与血清中的相对表达量呈正相关(r=0.459,P<0.05)。血清miR-34a-5p诊断宫颈癌的ROC曲线下面积为0.860(95%CI:0.803~0.917)。[结论] HR-HPV宫颈癌患者血清miR-34a-5p下调,与癌组织miR-34a-5p表达呈正相关,有望成为宫颈癌诊断的分子标志物。

[Objective] To investigate the expression of miR-34a-5p in tissues and serum of cervical cancer patients with high risk human papillomavirus(HR-HPV) infection and its clinical significance.[Methods] The expression of miR-34a-5p in cervical cancer tissues and adjacent tissues of 70 HRHPV-positive patients was detected by RT-PCR. The expression of miR-34a-5p in serum samples of100 HR-HPV-positive cervical cancer patients,80 HR-HPV positive cervical intraepithelial neoplasia(CIN) patients and 80 healthy subjects was also detected. Pearson correlation was used to analyze the correlation between the expression of miR-34a-5p in tissue and serum,and the relationship between mi R-34a-5p and the clinicopathological characteristics of cervical cancer was also analyzed. [Results]The relative expression of miR-34a-5p in cervical cancer tissues was significantly lower than that in adjacent tissues(P<0.05). The relative expression of miR-34a-5p in serum samples of cervical cancer patients was significantly lower than that of CIN patients and normal subjects(P<0.05). The relative expression of serum miR-34a-5p in CIN patients was significantly lower than that in normal subjects(P<0.05). The relative expression of tissue miR-34a-5p was correlated with lymph node metastasis(P<0.05).The relative expression of serum miR-34a-5p was correlated with lymph node metastasis and distant metastasis(P<0.05). The relative expression of miR-34a-5p in cancer tissue and serum was positively correlated with each other(r =0.459,P <0.05). The area under ROC curve of serum miR-34a-5p was0.860(95% CI:0.803 ~ 0.917). [Conclusion] The down-regulation of serum mi R-34a-5p in HR-HPV positive cervical cancer patients is positively correlated with the expression of miR-34a-5p in cancer tissues,which is expected to be a molecular marker for diagnosis of cervical cancer.