染井吉野樱的茎段培养与胚培养比较

Comparative Stem Culture and Embryo Culture of Cerasus yedoensis ‘Somei-yoshino’

以染井吉野樱为试材,比较茎段及种胚2种外植体在启动及增殖阶段的表现,探讨2种外植体的成苗效率以及不同植物生长调节剂对染井吉野樱芽诱导、不定芽增殖及生根的影响。结果表明:在MS+6-BA 2.0 mg·L^-1+IBA 0.1 mg·L^-1中腋芽诱导效果最好,诱导率可达61.29%。NAA对胚芽诱导的影响不显著,在MS+6-BA 2.0 mg·L^-1+IBA 0.05 mg·L^-1+NAA 0.05 mg·L^-1中种胚的诱导率可达86.02%。适宜的增殖培养基为MS+6-BA 2.0 mg·L^-1+IBA 0.1 mg·L^-1,腋芽苗及胚芽苗的平均增殖系数分别达到了3.73和4.09。在1/2MS+IBA 0.5 mg·L^-1+NAA 0.1 mg·L^-1中生根率可达70.97%。生根后的幼苗经过炼苗,移栽在腐殖质∶河沙∶蛭石体积比为2∶1∶1的基质中,成活率可达87.09%。

Cerasus yedoensis’Somei-yoshino’ was used as material,seeding efficiency of different explant by comparing the induction rate of stem with axillary bud and embryo which used as explant in single bud induction,cluster buds induction was imestigate.And the effect of different plant growth regulators on single bud induction,cluster buds induction and rotting was explored.The results showed that MS medium supplemented with 2.0 mg·L^-1 6-BA and 0.1 mg·L^-1 IBA showed the highest single bud induction rate(61.29%)using stem with axillary bud as explants.The influence of NAA for embryo induction was not significant.MS medium supplemented with 2.0 mg·L^-1 6-BA,0.05 mg·L^-1 IBA and 0.05 mg·L^-1 NAA showed the highest single bud induction rate(86.02%)using embryo as explants.MS medium with 2.0 mg·L^-1 6-BA and 0.1 mg·L^-1 IBA exhibited the highest clustered bud proliferation rate(3.73 and 4.09).1/2 MS medium with 0.5 mg·L^-1 IBA and 0.1 mg·L^-1 NAA was suitable for plantlet rooting,and the rooting rate was 70.97%.The survive rate was above 87.09% when the plantlets were due to be moved to the nursery.The medium with 50% humus,25% sand and 25% roseite was effective for rooting.