摘要
目的研究结肠肿瘤中高迁移率族蛋白B1基因(HMGB1)的差异表达及预后价值。方法从Oncomine及TCGA数据集中筛选出2191例结肠肿瘤患者HMGB1基因表达数据及临床病理数据,采用Mann-Whitney U检验比较结肠癌与腺瘤、左半结肠癌与右半结肠癌、原位癌与浸润癌、黏液性腺癌与其他病理类型结肠癌、以及发生淋巴结转移与无淋巴结转移、发生远处转移与无远处转移结肠癌组织中HMGB1基因差异表达情况,并绘制Kaplan-Meier生存曲线。结果HMGB1基因在结肠癌组织和腺瘤组织中均较正常结肠组织高表达(P<0.001),在结肠癌组织中较结肠腺瘤组织中高表达,在左半结肠癌组织中较右半结肠癌高表达(P<0.05),在黏液性腺癌组织中较其他病理类型低表达(P<0.05),在浸润癌组织中较原位癌高表达(P<0.001)。有淋巴结转移及远处转移者较未转移者高表达(P<0.05)。HMGB1基因高表达提示更高的5年生存率(P=0.011),尤其对于女性结肠癌患者(P=0.006)。结论HMGB1基因可作为判断结肠癌浸润深度、淋巴转移、远处转移及预后的标志物。
Objective To study the expression and prognosis of high mobility group protein B1(HMGB1)gene in colon tumor.Methods The HMGB1-related data of 2191 colon tumor patients were mined from Oncomine and TCGA datasets.The Mann-Whitney U test was used to compare the differential expression of HMGB1 between colonic adenoma and carcinoma,left and right half colon cancer,carcinoma in situ and infiltrating cancer,mucinous adenocarcinoma and other pathological types,with lymph node metastasis and without lymph node metastasis,with distant metastasis and without distant metastasis of colon cancer.Then the Kaplan Meier survival curve was drawn.Results HMGB1 gene was highly expressed in colon cancer tissues and adenoma tissues than in normal colon tissues(P<0.001),higher in colon cancer tissues than in colonic adenoma tissues,overexpressed in left colon cancer compared to right colon cancer(P<0.05),lower in mucinous adenocarcinoma of the colon than other pathological tissue types(P<0.05),overexpressed in infiltrating carcinoma compared with carcinoma in situ(P<0.001),overexpressed in patients with regional lymph node metastasis(P<0.05),and overexpressed in metastatic colon cancer than nonmetastatic colon cancer(P<0.05).Overexpression of HMGB1 gene indicates a higher 5-year survival rate(P=0.011),especially for female patients(P=0.006).Conclusion HMGB1 gene can be used as a marker to evaluate the invasion depth,lymphatic metastasis,distant metastasis and prognosis of colon cancer.
作者
苏献豪
王会鹏
陈文杰
沈家门
叶桃
蔡元坤
Su Xianhao;Wang Huipeng;Chen Wenjie;Shen Jiamen;Ye Tao;Cai Yuankun(Department of General Surgery,Minhang Hospital,Fudan University,Shanghai 201199,China;Department of General Surgery,Shanghai Fifth People's Hospital,Fudan University,Shanghai 200433,China)
出处
《中华结直肠疾病电子杂志》
2020年第6期552-557,共6页
Chinese Journal of Colorectal Diseases(Electronic Edition)
基金
上海市科学技术委员会科研课题项目(No.17411967600)
2015年上海领军人才“后备队”(闵行领军人才)资助
闵行区医疗系统大学科建设项目(No.2017MWDXK01)。
