紫杉醇-介孔氧化铜纳米球给药体系的制备及对肝癌细胞SMMC-7721作用

Preparation of Paclitaxel-Mesoporous Copper Oxide Nanospheres Drug Delivery System and Its Effect on Hepatoma Cell SMMC-7721

目的构建介孔氧化铜纳米球(mesoporous copper oxide nanospheres,MCON)负载紫杉醇(paclitaxel,PTX)纳米药物传递系统(MCON-PTX),考察其对SMMC-7721肝癌细胞抑制效果。方法以介孔碳纳米球为模板,氯化铜为铜源,采用硬模板法制备介孔氧化铜纳米球,然后通过吸附法负载紫杉醇(PTX)得到MCON-PTX。通过扫描电子显微镜、透射电子显微镜和差示扫描量热法对载药系统进行表征,体外溶出实验考察PTX释放行为,细胞实验考察MCON-PTX对SMMC-7721肝癌细胞的作用效果。结果MCON的粒径为230 nm,载药量为(13.65±0.725)%。体外溶出结果表明,MCON显著改善了PTX的溶出速率。细胞实验表明MCON-PTX显著改善了PTX的肿瘤细胞抑制作用。结论MCON的介孔结构改善了难溶性药物PTX的水溶性,是一个具有潜力的理想难溶性药物载体材料。

Objective To build paclitaxel-mesoporous copper oxide nanospheres(MCON-PTX)drug delivery system and investigate its inhibitory effect on SMMC-7721 hepatoma cells.Methods Mesoporous carbon nanospheres was used as template and copper chloride as copper source.Mesoporous copper oxide nanospheres were prepared by hard template method,and MCON-PTX was then obtained by loading paclitaxel(PTX)by adsorption.The drug delivery system was characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM)and differential scanning calorimetry(DSC).The release behavior of PTX was investigated by in vitro dissolution test,and the effect of MCON-PTX on SMMC-7721 hepatoma cells was investigated by cell experiment.Results The particle size of MCON was 230 nm and the drug loading capacity was(13.65±0.725)%.The results of in vitro dissolution showed that MCON significantly improved the dissolution rate of PTX.Cell experiments showed that MCON-PTX significantly improved the tumor cell inhibitory effect of PTX.Conclusion The mesoporous structure of MCON improves the water solubility of PTX,an insoluble drug,which is an ideal insoluble drug carrier material with potential.