HPLC法同时测定吲哚美辛呋喃唑酮栓中吲哚美辛和呋喃唑酮的含量

Determination of Two Main Ingredients in Indometacin and Furazolidone Suppositories by HPLC

目的:建立HPLC法同时测定吲哚美辛呋喃唑酮栓中吲哚美辛和呋喃唑酮的含量。方法:色谱柱为Inertsil ODS-3(250 mm×4.6 mm,5μm);以乙腈-0.1 mol·L-1冰醋酸溶液(50∶50)为流动相;流速为1.0 ml·min-1;检测波长为320 nm;进样体积为20μl。结果:吲哚美辛的浓度范围为0.0020~0.5000 mg·ml-1时,峰面积与浓度呈现良好的线性关系(r=0.9999),加样回收率为100.3%,RSD=1.0%(n=9);呋喃唑酮的浓度范围为0.0030~0.6000 mg·ml-1时,峰面积与浓度呈现良好的线性关系(r=0.9992),加样回收率100.9%,RSD=0.9%(n=9)。结论:本方法准确、快速、简便,可用于吲哚美辛呋喃唑酮栓的质量控制。

Objective: To establish an HPLC method for the simultaneous determination of indometacin and furazolidone in indometacin and furazolidone suppositories. Methods: An Inertsil ODS-3( 250 mm×4.6 mm,5 μm) column was used for the separation with acetonitrile and 0.1 mol·L-1 acetic acid( 50 ∶ 50) as the mobile phase at the flow rate of 1.0 ml·min-1.The detection wavelength was320 nm. and the injection volume was 20 μl.Results: The linear range of indometacin and furazolidone was 0.002 0-0.500 0 mg·ml-1( r= 0.999 9) and 0.003 0-0.600 0 mg·ml-1( r = 0.999 2),respectively. The average recovery of indometacin and furazolidone was100.3%( RSD = 1.0%) and 100.9%( RSD = 0.9%)( n = 9),respectively. Conclusion: The method is simple and accurate,and can be used for the determination of two main ingredients in indometacin and furazolidone suppositories.