液相色谱-串联质谱法测定化妆品中谷胱甘肽和乙酰基六肽-8的含量

Determination of the Contents of Glutathione and Acetyl Hexapeptide-8 in Cosmetics by LC-MS/MS

建立了以液相色谱-串联质谱法(LC-MS/MS)测定化妆品中谷胱甘肽和乙酰基六肽-8含量的方法。样品经水提取,用Agilent Poroshell 120 EC-C18柱(4.6 mm×100 mm×2.7μm)分离,以乙腈和含有体积分数0.1%乙酸水溶液等度洗脱,流速0.3 mL/min,以多反应模式(MRM)监测,外标法定量。结果表明,谷胱甘肽和乙酰基六肽-8在1~200 ng/mL范围内线性良好,相关系数(r)均大于0.995,检出限(LOD)分别为0.08μg/g和0.15μg/g,平均回收率为85.1%~105.6%,相对标准偏差(RSD,n=6)为0.5%~4.4%。该方法快速简便、灵敏度高,可用于化妆品中谷胱甘肽和乙酰基六肽-8的快速准确检测。

A LC-MS/MS method was established for the determination of the contents of glutathione and acetyl hexapeptide-8 in cosmetics.The samples were extracted with water,the Agilent reversed phase column Poroshell 120 EC C18(4.6 mm×100 mm×2.7μm)was used with acetonitrile-0.1%(volume fraction)acetic acid aqueous solution as the mobile phase.The flow rate was 0.3 mL/min,the multiple reaction monitor mode was performed,external standard quantification was conducted.The results showed that it had a good linearity in the range of 1-200 ng/mL(r>0.995).The detection limits of glutathione and acetyl hexapeptide-8 were 0.08μg/g and 0.15μg/g.The spiked recoveries were 85.1%to 105.6%with the relative standard deviation of 0.5%to 4.4%.The method is simple,rapid and highly sensitive.It is suitable for the determination of the contents of glutathione and acetyl hexapeptide-8 in cosmetics.