期刊文献+

分子生物学方法在环境微生物生态学中的应用研究进展 被引量:3

Application of Molecular Biological Methods in Environmental Microbial Ecology
下载PDF
导出
摘要 随着分子生物学的发展和完善,微生物在生态系统中的作用越来越受到重视。目前,先进的分子生物学和物理技术的迅速发展已应用于环境微生物多样性等。生态微生物研究的主要目标为确定微生物的类型和多样性,然而,确定在一段时间内有效的微生物的定义以及微生物的作用或潜在影响,由于微生物学研究方法的复杂性和多样性,不知道如何选择这些方法。主要介绍和分析微生物响应分子生物学方法的多样性和功能。近年来,微生物学和单细胞水平的研究方法(如纳米二次离子质谱)为研究人员选择合适的研究方法来研究环境微生物提供了依据。 With the development and improvement of molecular biology,more and more attention has been paid to the role of microorganisms in the ecosystem.At present,the rapid development of advanced molecular biology and physical technology has been applied to environmental microbial diversity.Generally speaking,the main goal of the study of ecological microorganisms is to determine the types and diversity of microorganisms.However,to determine the definition of microorganisms that are effective over a period of time and their roles or potential impacts,researchers do not know how to choose these methods due to the complexity and diversity of microbiological research methods.This paper will introduce and analyze the diversity and function of microbial response molecular biological methods.In recent years,the research methods of Microbiology and single cell level(such as nano secondary ion mass spectrometry)provide the basis for researchers to select appropriate research methods to study environmental microorganisms.
作者 高乐 Gao Le
出处 《化工设计通讯》 CAS 2020年第12期85-86,共2页 Chemical Engineering Design Communications
关键词 微生物 单细胞水平法 分子生物学 微生物生态学 应用 研究进展 microorganism single cell level method molecular biology microbial ecology application research progress
  • 相关文献

参考文献4

二级参考文献23

  • 1周启星,宋玉芳,孙铁珩.生物修复研究与应用进展[J].自然科学进展,2004,14(7):721-728. 被引量:30
  • 2邢德峰,任南琪.应用DGGE研究微生物群落时的常见问题分析[J].微生物学报,2006,46(2):331-335. 被引量:82
  • 3余素林,吴晓磊,钱易.环境微生物群落分析的T-RFLP技术及其优化措施[J].应用与环境生物学报,2006,12(6):861-868. 被引量:67
  • 4[1]Ward DM,Weller R,Bateson MM.16SrRNA sequences reveal numerous uncultured microorganisms in a natural community[J].Nature,1990,345:3673-3682.
  • 5[2]Myers RM,Fischer SG,Lerman LS,et al.Nearly all single base substitutions in DNA fragment joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis[J].Nucleic Acids Res,1985,13:3131-45.
  • 6[3]Muyzer G,de Weel EC,Uittrlindern AG.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction -amplified genes coding for 16SrRNA[J].Appl Environ Microbiol,1993,59(3):695-700.
  • 7[5]Vincent D,Roy D,Mondon F,et al.Characterization of bifidobacteria by random DNA amplification[J].Int J Food Microbiol,1998,43(3):185-193.
  • 8[6]Marco Ventura,Douwe van Sindaren,Gerald F.Fitzgerald,et al.Insights into the taxonomy,genetics and physiology of bifidobacteria[J].Antonie van Leeuwenhoek,2004,86:205-223.
  • 9[7]Hopkins KL,Desai M,Frost J A,et al.Fluorescent amplified fragment length polymorphism genotyping of Campylobater jejuni and Campylobacter coli strains and relationship with host specificity,serotyping,and phage typing[J].Clin Microbiol,2004,42:229-235.
  • 10[8]Denis Roy,PierreWard,Daniel Vincent,et al.Molecular Identification of Potentially Probiotic Lactobacilli[J].Current Microbiology,2000,40:40-46.

共引文献29

同被引文献74

引证文献3

二级引证文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部