紫娟茶花青素提取物抑制酪氨酸酶活性研究

Study on Inhibitory Effect of Anthocyanin Extract from Zi juan Tea on Tyrosinase Activity

利用高效液相色谱分析测定了紫娟茶花青素提取物中飞燕草色素、矢车菊素、芍药素、天竺葵色素、飞燕草色素-3-O-半乳糖苷、矢车菊素-3-半乳糖苷六种花青素成分的含量。以左旋多巴(L-DOPA)为底物,应用Flex Station 3读板器测定了紫娟茶花青素提取物抑制酪氨酸酶能力,IC 50值为1.309 mg/mL,表明紫娟茶花青素对酪氨酸酶具有很强的抑制作用。

Six active components in Zi juan tea were determined by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS).The analysis of retention time and molecular fragment ion peak showed that Zi juan tea contained six kinds of anthocyanins:Delphinidin,Cyanidin,Peonidin,Pelargonidin,Delphinidin-3-O-galactoside and Cyanidin-3-galactoside.Using levodopa(L-DOPA)as substrate,the inhibitory effect of anthocyanin extract from Zi juan tea on tyrosinase was determined by Flex Station 3 reader.The IC 50 value was 1.309 mg/mL,which indicated that anthocyanin from Zi juan tea had a strong inhibitory effect on tyrosinase.