苦草对底泥中重金属铜的生理响应

Physiological Response of Vallisneria Asiatica to Heavy Metal Copper in Sediment

随着工农业发展,大量重金属被废弃,对湖泊、海洋、土壤等环境造成了严重污染,引发了很多重金属污染环境问题。为研究苦草对底泥中重金属铜的生理响应,实验设置水深1.5m,底泥中重金属铜浓度分别设对照组(不添加)、50mg/kg、80mg/kg、150mg/kg、250mg/kg,分析Cu^2+浓度对苦草叶片中抗氧化酶(超氧化物歧化酶(SOD),过氧化物酶(POD))比活力、可溶性蛋白含量和叶绿素含量的影响。结果表明,在铜胁迫下苦草叶片中的抗氧化酶活性呈先上升后下降的趋势,底泥中铜浓度高达250mg/kg时,叶绿素含量下降,Cu^2+浓度为80mg/kg时,蛋白质含量提高。实验发现,低浓度铜含量会促进苦草的生长,当Cu^2+浓度增加到150mg/kg对苦草产生一定的胁迫作用。旨在为近年来鄱阳湖水生态系统沉水植物的保护提供依据,并为进一步探索沉水植物对重金属污染的早期预警和耐性机理提供参考。

With the development of industry and agriculture,a large number of heavy metals have been abandoned,causing serious pollution to lakes,oceans,soil and other environmentstriggering a lot of heavy metal pollution problems.For the research of physiological response of Vallisneria Asiatica to heavy metal copper in sediment,the water depth is 1.5m in the experimentcopper concentrations of heavy metals in sediment were set in the control group(not adding),50 mg/kg,80 mg/kg,150 mg/kg and 250 mg/kg,to analyze the effects of Cu^2+concentration on the analyze the effects of Cu^2+concentration on the activity of antioxidant enzymes(superoxide dismutase(SOD),peroxidase(POD)),soluble protein content and chlorophyll content in Vallisneria natans leaft.The results showed that under the copper stress,the activity of antioxidant enzymes in the leaves of the plant showed a trend of first increasing and then decreasing.The content of chlorophyll decreased when the concentration of copper in the bottom was as high as 250mg/kg,while the content of protein increased when the concentration of Cu^2+was 80mg/kg.It was found that the low concentration of copper promoted the growth of Vallisneria Asiatica,and when the concentration of copper increased to 150mg/kg,the Vallisneria Asiatica were subjected to certain stress.The aim is to provide the basis for the protection of submerged plants in poyang Lake water ecosystem in recent years,and to provide reference for the further exploration of the mechanism of early warning and tolerance of submerged plants to heavy metal pollution.