云南分心木总黄酮提取及抗氧化和对脂肪变性L02肝细胞的作用

Optimization of extraction technology of general flavone from Yunnan diaphragma juglandis fructus and its antioxidant activities and effects on lipid-changed L02 hepatocytes

采用单因素结合响应面试验优化云南分心木总黄酮提取工艺,研究分心木总黄酮提取物清除过氧化氢(H2O2)、超氧阴离子自由基(O2^-·)、羟基自由基(·OH)和DPPH自由基(DPPH·)的能力和降低脂肪变性L02肝细胞内总胆固醇(TC)和甘油三酯(TG)的作用。结果表明:云南分心木总黄酮最佳提取工艺为乙醇浓度55%、提取温度80℃、提取时间80min,该条件下总黄酮提取得率为9.88%;25μg/mL分心木总黄酮对DPPH·的清除率为77.87%;1000μg/mL清除H2O2、O2^-·、·OH分别为89.39%,89.56%,88.04%;与模型组相比,300,400μg/mL分心木总黄酮均显著降低脂变L02肝细胞TC和TG含量。云南分心木总黄酮具有较高的体外抗氧化活性及显著降低脂肪变性L02肝细胞TG和TC活性。

The extraction process of the general flavonoids from Diaphragma juglandis Fructus(DJF)in Yunnan was optimized.and the ability of the general flavonoids extracts to scavenge hydrogen peroxide(H2O2),superoxide anion free radicals(O2^-·),hydroxyl free radicals(·OH)and DPPH free radicals(DPPH·)and the role of reducing fatty degeneration L02 liver intracellular total cholesterol(TC)and triglycerides(TG)were investigated.The results showed that the best extraction process for general flavonoids from Yunnan DJF was extraction with 55%ethanol at 80℃for 80 min.Under the control of these conditions,the extraction yield of general flavonoids was 9.88%;25μg/mL general flavonoids from DJF removed 77.87%of DPPH;89.39%,89.56%,and 88.04%of H2O2,O2^-·and·OH were removed by 1000μg/mL of general flavonoids.Compared with the model group,300μg/mL and 400μg/mL general flavonoids from DJF significantly reduced the content of TC and TG in steatosis L02 hepatocytes.The general flavone of DJF from Yunnan present high antioxidant activity and reduced TG and TC activities of steatosis L02 hepatocytes.