149株布鲁氏菌病原快速诊断方法的评价

Evaluation of rapid diagnostic tests for 149 isolates of Brucella species

目的评价细菌培养快速准确诊断布鲁氏菌的方法。方法采用细菌生长曲线、革兰染色、石碳酸复红单染、尿素、氧化酶、触酶等快速实验方法分离的149株布鲁氏菌与疾病控制中心(CDC)标准试管凝集法(STA)结果进行比较,对实验室诊断方法进行评价。结果分离并确诊的149株布鲁氏菌中,有2株的STA结果为阴性。结论实验室快速诊断布鲁氏菌的方法准确性高、易操作、生物安全性高,不易漏诊。

The diagnosis of Brucella infections can be made by culture,which can be identified by diverse rapid diagnostic tests.In this study,149 Brucella isolates that was identified by rapid diagnostic tests including conventional growth curves,Gram stain,carbolfuchsin stain,urea tests,oxidase tests and catalase tests,were compared with CDC’s standard agglutination test(SAT)results.Among the 149 Brucella spp.isolated and confirmed by rapid diagnostic tests,two isolates were negative in SAT.Therefore,this observation suggests that rapid diagnostic tests for Brucella spp.is of high accuracy,ease in operation,considerable biosafety and free of misdiagnosis.