海南省4个历史疫情市县猪链球菌Ⅱ型血清学抽样检测

Serological Sampling Test of Streptococcus suis SerotypeⅡin Four Historically Infected Counties/Cities in Hainan Province

为了解海南省历史疫区猪链球菌Ⅱ型的流行及分布情况,选择东方、文昌、儋州及白沙4个历史疫情市县,针对中小规模养殖场及散养户开展了血清学调查和生物安全问卷调查。本次调查共涉及4个市县的29个养殖场点,包含中小规模场9个、散养户20个;共检测猪血清样品307份,检出阳性95份,个体阳性检出率为30.94%(95/307),检出阳性场点17个,群体阳性检出率为58.62%(17/29)。4个市县中,群体阳性检出率介于28.57%~85.71%,个体阳性检出率介于4.00%~84.15%,不同市县间差异均显著(P<0.05);不同场点中,中小规模场的群体阳性检出率(77.78%)高于散养户(50.00%),但个体阳性检出率(28.29%)低于散养户(36.27%),差异均显著(P<0.05)。被调查养殖场户的生物安全状况不佳,35.7%的场点人员随意进出,仅21.4%的场点设有车辆出入消毒设施,定期组织培训的场点仅占21.4%。结果表明,海南省历史疫情市县的猪链球菌Ⅱ型污染面广,防控效果不理想,疫情传播风险较高,尤其是疫情多发地区和散养户。建议政府主管部门加大防控力度,加强养殖人员技术培训和指导,提升养殖场点生物安全水平,减少疫情发生。本调查为今后海南省猪链球菌病防控提出了可行性指导意见。

In order to identify the prevalence and distribution of Streptococcus suis serotypeⅡin the historically infected regions in Hainan Province,four counties/cities including Dongfang,Wenchang,Danzhou and Baisha were selected,and an serological investigation and a questionnaire survey were carried out in 29 farms/premises covering 9 small-medium scale farms and 20 free-range households.The results showed that 95 out of 307 serum samples were detected positive,with an individual positive rate of 30.94%(95/307),17 positive farms/premises were detected out with a population positive rate of 58.62%(17/29).The population positive detection rate ranged from 28.57%to 85.71%,and the individual rate was in the range of 4.00%to 84.15%,both of which were obviously different in various counties/cities(P<0.05);the small-medium farms were with higher population positive detection rate(77.78%)than free-range households(50.00%),but with lower individual positive detection rate(28.29%)than free-range households(36.27%),both of which were obviously different(P<0.05).The bio-security status of the farms and households concerned was poor,in which,35.7%were freely accessed to,only 21.4%were equipped with disinfection facilities for vehicles,and only 21.4%farms regularly organized training.It was concluded that Streptococcus suis serotypeⅡwas widely spread in the historically infected regions in Hainan Province,with poor effect of prevention and control and high risk of spreading,especially in the epidemic regions and free-range households.It was suggested that government authorities should strengthen the prevention and control,improve technical training and guidance for breeding workers,and upgrade the bio-security level of farms/premises,so as to reduce any outbreak.In the study,feasible guidance was provided for the prevention and control of Streptococcus suis in Hainan Province in the future.