荷叶提取液的性能探究

Research on the performance of lotus leaf extract

目的对荷叶提取液的性能进行探究。方法通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine,DPPH)清除自由基实验、抑菌实验和鸡胚绒毛尿囊膜实验对荷叶提取液的抗氧化性、抑菌性和低刺激性能进行探究。结果荷叶提取液具有抗氧化的性能,自由基清除率为50%时所需荷叶提取液浓度为0.00957 g/mL;对金黄色葡萄球菌、大肠杆菌的最小抑菌浓度为0.2 g/mL,对黑曲霉菌和绿脓杆菌的最小抑菌浓度为0.5g/mL,对酵母菌的最小抑制浓度为0.5g/mL;0.2g/mL以下浓度的荷叶提取液无刺激作用。结论荷叶提取液具有抗氧化性、抑菌和低刺激性。

Objective To explore the performance of lotus leaf extract. Methods The antioxidant,antibacterial and low irritation properties of lotus leaf extract were explored by scavenging free radical,antibacterial experiment and chicken embryo chorioallantoic membrane experiment with DPPH(1,1-diphenyl-2-trinitropheny lhydrazine). Results The lotus leaf extract had antioxidant properties. Extracts of lotus leaf alkaloid with 0.00957 g/mL eliminates 50% free radical. The minimum inhibitory concentration of lotus leaf alkaloid against Staphylococcus aureus and Escherichia coli was 0.2 g/mL,against Pseudomonas aeruginosa and Aspergillus niger was 0.5 g/mL,and the minimum inhibitory concentration of yeast was 0.5 g/mL. Extract of lotus leaf alkaloid under 0.2 g/mL was not irritant. Conclusion The lotus leaf alkaloid extract has antioxidant,antibacterial and low irritation properties.