不同基质奶粉中泛酸检测方法对比

Comparison of detection methods of pantothenic acid in different matrix milk powder

目的对比不同基质奶粉中泛酸检测方法,以探究针对不同奶粉基质中泛酸检测最适方法和最适条件。方法以不含水解蛋白奶粉和水解蛋白奶粉为研究对象,分别采用GB5009.210-2016《食品安全国家标准食品中泛酸的测定》中高效液相色谱法和微生物法进行检测,评价2种方法的优缺点,选择最适检测方法。结果对于高效液相色谱法,优化色谱条件、更换色谱柱、沉淀蛋白、PH值等几个方面的调整,均无法减少杂峰对目标峰的干扰作用,仅调整流动相比例可以实现目标锋和杂峰的分离,但是该方法耗时长、无规律,对仪器设备耗材和人员均造成部分程度浪费,且重复率低,不宜采用;微生物法针对不同基质奶粉(包含水解蛋白奶粉)检测准确性和稳定性均符合方法要求。结论针对大部分奶粉(水解蛋白基质除外)高效液相色谱法和微生物法检测一致性较好,精密度、准确性等均符合要求;针对水解蛋白奶粉,微生物法检测准确性和稳定性较高效液相色谱法好。

Objective To compare the detection methods of pantothenic acid in different matrix milk powder,in order to explore the optimal method and conditions for the detection of pantothenic acid in different milk powder matrix.Methods Milk powder without hydrolyzed protein and milk powder with hydrolyzed protein were taken as research object.High performance liquid chromatography(HPLC)and microbiological method in GB 5009.210-2016 National food safety standard-Determination of pantothenic acid were used for detection.The advantages and disadvantages of the 2 methods were evaluated,and the optimal detection method was selected.Results For high performance liquid chromatograph,adjustment from the optimization of chromatographic conditions,replacement of chromatographic column,precipitation of protein,pH value and other aspects could not reduce the interference of miscellaneous peaks on the target peak.Only adjusting the proportion of mobile phase could achieve the separation of target peak and impurity peak.However,this method was time-consuming and irregular,would cause partial waste of equipment,consumables and personnel to a certain extent,and the repetition rate was low,so it was not suitable to use.The accuracy and stability of microbiological method for different matrix milk powder(including hydrolyzed protein milk powder)could meet the requirements of the method.Conclusion For most milk powder(except hydrolyzed protein matrix),high performance liquid chromatography and microbiological method have good consistency,the precision and accuracy meet the requirements;For hydrolyzed protein milk powder,the accuracy and stability of microbiological method are better than those of high performance liquid chromatography.